Lee, Junwoo Yoo, Yong Kyoung Lee, Dohwan Kim, Cheonjung Kim, Kang Hyeon Lee, Seungmin Kwak, Seungmin Kang, Ji Yoon Kim, Hyungsuk Yoon, Dae Sung Hur, Don Lee, Jeong Hoon 2024-01-19T15:04:20Z 2024-01-19T15:04:20Z 2021-09-04 2021-03-07 1473-0197 https://pubs.kist.re.kr/handle/201004/117269 Ion concentration polarization (ICP) is one of the preconcentration techniques which can acquire a high preconcentration factor. Still, the main hurdles of ICP are its instability and low efficiency under physiological conditions with high ionic strength and abundant biomolecules. Here, we suggested a sequentially driven ICP process, which enhanced the electrokinetic force required for preconcentration, enabling enrichment of highly ionic raw samples without increasing the electric field. We acquired a 13-fold preconcentration factor (PF) in human serum using a paper-based origami structure consisting of multiple layers for three-dimensional sequential ICP (3D seq-ICP). Moreover, we demonstrated a paper-based enzyme-linked immunosorbent assay (ELISA) by 3D seq-ICP using tau protein, showing a 6-fold increase in ELISA signals. English Royal Society of Chemistry Origami paper-based sample preconcentration using sequentially driven ion concentration polarization Article 10.1039/d0lc01032d 1 Lab on a Chip, v.21, no.5, pp.867 - 874 Lab on a Chip 21 5 867 874 scie scopus 000626710700002 2-s2.0-85102289214 Biochemical Research Methods Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Instruments & Instrumentation Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Instruments & Instrumentation Article