Bong, Ji-Hong Kim, Tae-Hun Jung, Jaeyong Lee, Soo Jeong Sung, Jeong Soo Lee, Chang Kyu Kang, Min-Jung Kim, Hyun Ok Pyun, Jae-Chul 2024-01-19T15:05:08Z 2024-01-19T15:05:08Z 2021-10-21 2021-03 1976-0280 https://pubs.kist.re.kr/handle/201004/117312 Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid. English KOREAN BIOCHIP SOCIETY-KBCS Competitive Immunoassay of SARS-CoV-2 Using Pig Sera-Derived Anti-SARS-CoV-2 Antibodies Article 10.1007/s13206-021-00011-6 1 BIOCHIP JOURNAL, v.15, no.1, pp.100 - 108 BIOCHIP JOURNAL 15 1 100 108 scie scopus kci ART002695618 000618961800003 2-s2.0-85101142280 Biochemical Research Methods Chemistry, Analytical Nanoscience & Nanotechnology Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Article Competitive assay SARS-CoV-2 Culture fluid Anti-SARS-CoV-2 nucleoprotein antibody Nucleoprotein (NP)