Lee, Soojin Lee, Hyun Su Chung, Justin J. Kim, Soo Hyun Park, Jong Woong Lee, Kangwon Jung, Youngmee 2024-01-19T15:05:25Z 2024-01-19T15:05:25Z 2021-09-05 2021-03 1661-6596 https://pubs.kist.re.kr/handle/201004/117328 A flexible and bioactive scaffold for adipose tissue engineering was fabricated and evaluated by dual nozzle three-dimensional printing. A highly elastic poly (L-lactide-co-epsilon-caprolactone) (PLCL) copolymer, which acted as the main scaffolding, and human adipose tissue derived decellularized extracellular matrix (dECM) hydrogels were used as the printing inks to form the scaffolds. To prepare the three-dimensional (3D) scaffolds, the PLCL co-polymer was printed with a hot melting extruder system while retaining its physical character, similar to adipose tissue, which is beneficial for regeneration. Moreover, to promote adipogenic differentiation and angiogenesis, adipose tissue-derived dECM was used. To optimize the printability of the hydrogel inks, a mixture of collagen type I and dECM hydrogels was used. Furthermore, we examined the adipose tissue formation and angiogenesis of the PLCL/dECM complex scaffold. From in vivo experiments, it was observed that the matured adipose-like tissue structures were abundant, and the number of matured capillaries was remarkably higher in the hydrogel-PLCL group than in the PLCL-only group. Moreover, a higher expression of M2 macrophages, which are known to be involved in the remodeling and regeneration of tissues, was detected in the hydrogel-PLCL group by immunofluorescence analysis. Based on these results, we suggest that our PLCL/dECM fabricated by a dual 3D printing system will be useful for the treatment of large volume fat tissue regeneration. English MDPI SMALL-INTESTINAL SUBMUCOSA MESENCHYMAL STEM-CELLS POROUS SCAFFOLD DESIGN EXTRACELLULAR-MATRIX DEGRADATION BEHAVIOR IN-VITRO BIOMATERIAL BONE INFLAMMATION ANGIOGENESIS Enhanced Regeneration of Vascularized Adipose Tissue with Dual 3D-Printed Elastic Polymer/dECM Hydrogel Complex Article 10.3390/ijms22062886 1 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, no.6, pp.1 - 22 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22 6 1 22 scie scopus 000645698100001 2-s2.0-85102296226 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article SMALL-INTESTINAL SUBMUCOSA MESENCHYMAL STEM-CELLS POROUS SCAFFOLD DESIGN EXTRACELLULAR-MATRIX DEGRADATION BEHAVIOR IN-VITRO BIOMATERIAL BONE INFLAMMATION ANGIOGENESIS 3d printing PLCL decellularization angiogenesis dECM hydrogel adipose tissue regeneration