Park, Jun-Hee Bong, Ji-Hong Jung, Jaeyong Sung, Jeong Soo Lee, Ga-Yeon Kang, Min-Jung Pyun, Jae-Chul 2024-01-19T15:05:36Z 2024-01-19T15:05:36Z 2021-09-04 2021-03 0141-0229 https://pubs.kist.re.kr/handle/201004/117340 In this work, we present a novel microbial biosensor for Salmonella based on impedance spectrometry by using isolated antibodies against a specific bacterial strain from human serum. Anti-Salmonella (or BL21(DE3)) antibodies were isolated from human serum using S. enteritidis (or BL21(DE3)) and the mutant strain ClearColi. After the purification steps, the purification yield of the antibodies was calculated to be 0.2 %. From the FACS analysis, the isolated anti-Salmonella antibodies were estimated to have more than 6-fold higher binding affinity for S. enteritidis compared to antibodies against other kinds of Gram-negative bacterial strains, including HB101, ClearColi, JM110, DH5 alpha, and BL21(DE3). Finally, the anti-Salmonella antibodies isolated herein were used for bacterial detection using electrochemical biosensors based on impedance spectrometry and the R-ct value of the antibodies was estimated for S. enteritidis from the Nyquist plot. The limit of detection of the isolated anti-Salmonella antibodies was estimated to be 1.0 x 10(3) cells/mL for S. enteritidis and 1.0 x 10(6) cells/mL for BL21 (DE3), respectively. English ELSEVIER SCIENCE INC Microbial biosensor for Salmonella using anti-bacterial antibodies isolated from human serum Article 10.1016/j.enzmictec.2020.109721 1 ENZYME AND MICROBIAL TECHNOLOGY, v.144 ENZYME AND MICROBIAL TECHNOLOGY 144 scie scopus 000618543000007 2-s2.0-85098672711 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article Anti-Salmonella antibody Bacterial detection ClearColi Impedance spectrometry