Kim, Hyungi Choi, Sang-Kee Ahn, Jungmo Yu, Hojeong Min, Kyoungha Hong, Changgi Shin, Ik-Soo Lee, Sanghee Lee, Hakho Im, Hyungsoon Ko, JeongGil Kim, Eunha 2024-01-19T15:31:01Z 2024-01-19T15:31:01Z 2021-09-02 2021-02-15 0925-4005 https://pubs.kist.re.kr/handle/201004/117402 Multiplexed analysis allows simultaneous measurements of multiple targets, improving the detection sensitivity and accuracy. However, highly multiplexed analysis has been challenging for point-of-care (POC) sensing, which requires a simple, portable, robust, and affordable detection system. In this work, we developed paper-based POC sensing arrays consisting of kaleidoscopic fluorescent compounds. Using an indolizine structure as a fluorescent core skeleton, named Kaleidolizine (KIz), a library of 75 different fluorescent KIz derivatives were designed and synthesized. These KIz derivatives are simultaneously excited by a single ultraviolet (UV) light source and emit diverse fluorescence colors and intensities. For multiplexed POC sensing system, fluorescent compounds array on cellulose paper was prepared and the pattern of fluorescence changes of KIz on array were specific to target chemicals adsorbed on that paper. Furthermore, we developed a machine-learning algorithm for automated, rapid analysis of color and intensity changes of individual sensing arrays. We showed that the paper sensor arrays could differentiate 35 different volatile organic compounds using a smartphone-based handheld detection system. Powered by the custom-developed machine-learning algorithm, we achieved the detection accuracy of 97 % in the VOC detection. The highly multiplexed paper sensor could have favorable applications for monitoring a broad-range of environmental toxins, heavy metals, explosives, pathogens. English ELSEVIER SCIENCE SA COLOR-DIFFERENCE FORMULA DISCOVERY BRIGHT DYES FLUOROPHORES INDOLIZINE SENSORS PROBES CELL Kaleidoscopic fluorescent arrays for machine-learning-based point-of-care chemical sensing Article 10.1016/j.snb.2020.129248 1 SENSORS AND ACTUATORS B-CHEMICAL, v.329 SENSORS AND ACTUATORS B-CHEMICAL 329 scie scopus 000612043900010 2-s2.0-85097473556 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article COLOR-DIFFERENCE FORMULA DISCOVERY BRIGHT DYES FLUOROPHORES INDOLIZINE SENSORS PROBES CELL Indolizine Fluorescent compound array Pattern recognition Machine learning Multiplexing