Lee, Jeonghyun Park, Hyosung Lim, Jiwon Jin, Hyung-Seung Park, Yoon Jung, Yu-Jin Ko, Hyun-Jeong Yoon, Sung-Il Lee, Geun-Shik Kim, Pyeung-Hyeun Choi, Sun Shim Xiao, Changchun Kang, Seung Goo 2024-01-19T15:31:29Z 2024-01-19T15:31:29Z 2021-09-02 2021-02-01 0022-1767 https://pubs.kist.re.kr/handle/201004/117429 B cells in the germinal center (GC) are programmed to form plasma cells (PCs) or memory B cells according to signals received by receptors that are translated to carry out appropriate activities of transcription factors. However, the precise mechanism underlying this process to complete the GC reaction is unclear. In this study, we show that both genetic ablation and pharmacological inhibition of glycogen synthase kinase 3 (GSK3) in GC B cells of mice facilitate the cell fate decision toward PC formation, accompanied by acquisition of dark zone B cell properties. Mechanistically, under stimulation with CD40L and IL-21, GSK3 inactivation synergistically induced the transcription factors Foxo1 and c-Myc, leading to increased levels of key transcription factors required for PC differentiation, including IRF4. This GSK3-mediated alteration of transcriptional factors in turn facilitated the dark zone transition and consequent PC fate commitment. Our study thus reveals the upstream master regulator responsible for interpreting external cues in GC B cells to form PCs mediated by key transcription factors. English AMER ASSOC IMMUNOLOGISTS FOLLICULAR HELPER-CELLS TRANSCRIPTION FACTOR C-MYC CENTER LIGHT RECEPTOR GENE SELECTION DIFFERENTIATION PROLIFERATION BACH2 GSK3 Restrains Germinal Center B Cells to Form Plasma Cells Article 10.4049/jimmunol.2000908 1 JOURNAL OF IMMUNOLOGY, v.206, no.3, pp.481 - + JOURNAL OF IMMUNOLOGY 206 3 481 + scie scopus 000610050700004 2-s2.0-85099902739 Immunology Immunology Article FOLLICULAR HELPER-CELLS TRANSCRIPTION FACTOR C-MYC CENTER LIGHT RECEPTOR GENE SELECTION DIFFERENTIATION PROLIFERATION BACH2