Park, Ye-Lim Choi, Tae-Rim Kim, Hyun Joong Song, Hun-Suk Lee, Hye Soo Park, Sol Lee Lee, Sun Mi Kim, Sang Hyun Park, Serom Bhatia, Shashi Kant Gurav, Ranjit Sung, Changmin Seo, Seung-Oh Yang, Yung-Hun 2024-01-19T15:31:47Z 2024-01-19T15:31:47Z 2022-01-10 2021-02 1017-7825 https://pubs.kist.re.kr/handle/201004/117444 Among various species of marine bacteria, those belonging to the genus Halomonas have several promising applications and have been studied well. However, not much information has been available on their antibiotic resistance. In our efforts to learn about the antibiotic resistance of strain Halomonas socia CKY01, which showed production of various hydrolases and growth promotion by osmolytes in previous study, we found that it exhibited resistance to multiple antibiotics including kanamycin, ampicillin, oxacillin, carbenicillin, gentamicin, apramycin, tetracycline, and spectinomycin. However, the H. socia CKY01 resistance pattern to kanamycin, gentamicin, apramycin, tetracycline, and spectinomycin differed in the presence of 10% NaCl and 1% NaCl in the culture medium. To determine the mechanism underlying this NaCl concentration-dependent antibiotic resistance, we compared four aminoglycoside resistance genes under different salt conditions while also performing time-dependent reverse transcription PCR. We found that the aph2 gene encoding aminoglycoside phosphotransferase showed increased expression under the 10% rather than 1% NaCl conditions. When these genes were overexpressed in an Escherichia coli strain, pETDuet-1::aph2 showed a smaller inhibition zone in the presence of kanamycin, gentamicin, and apramycin than the respective control, suggesting aph2 was involved in aminoglycoside resistance. Our results demonstrated a more direct link between NaCl and aminoglycoside resistance exhibited by the H. socia CKY01 strain. English KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY PALM BIOMASS HYDROLYSATE ANTIBIOTICS GROWTH ACID ACCUMULATION TOLERANCE OSMOLYTES STRESS NaCl Concentration-Dependent Aminoglycoside Resistance of Halomonas socia CKY01 and Identification of Related Genes Article 10.4014/jmb.2009.09017 1 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.31, no.2, pp.282 - 290 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 31 2 282 290 scie scopus kci ART002690410 000623844400014 2-s2.0-85102537418 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article PALM BIOMASS HYDROLYSATE ANTIBIOTICS GROWTH ACID ACCUMULATION TOLERANCE OSMOLYTES STRESS Halomonas strain antibiotic resistance salt tolerance genome sequencing