Min, Hyun Jin Park, Joon Soon Kim, Kyung Soo Park, Seung Yong Choi, Honghwan Seo, Ju Hee Kang, Miran Yoon, Joo-Heon Kim, Chang-Hoon Kim, Sehoon Cho, Hyung-Ju 2024-01-19T15:31:49Z 2024-01-19T15:31:49Z 2022-01-10 2021-02 0143-5221 https://pubs.kist.re.kr/handle/201004/117446 The function of high-mobility group box 1 (HMGB1) varies according to its location. However, the translocation mechanism behind HMGB1 remains unclear. We hypothesize that type 2 helper T cell (Th2) cytokines are involved in the translocation of HMGB1 in the upper airway epithelium. We investigated the mechanism behind HMGB1 translocation using Th2 cytokine stimulation and examined the clinical significance of HMGB1 translocation in allergic rhinitis (AR). Cytoplasmic and extracellular HMGB1 were increased in AR. Inhibiting HMGB1 translocation with glycyrrhizic acid (GA) decreased the level of antigen-specific immunoglobulin E (IgE), the degree of Periodic Acid-Schiff (PAS), and Sirius Red staining in the murine model. The in vivo reactive oxygen species (ROS) level in the nasal mucosa was higher in the mice with AR than in the controls. Th2 cytokine-induced up-regulation of the ROS and translocation of HMGB1 by Th2 cytokines was dependent on the generated ROS. The ROS level also increased in the murine model. We suggest that the Th2 cytokine-dual oxidase (DUOX)2-ROS-HMGB1 translocation axis is important in AR pathogenesis. English PORTLAND PRESS LTD GROUP BOX 1 MOBILITY GROUP BOX-1 OXIDATIVE STRESS CONTRIBUTES NEUTROPHILIC ASTHMA AIRWAY INFLAMMATION EPITHELIAL-CELLS HMGB1 PROTEIN CHILDREN BIOMARKER Th2 cytokines-DUOX2-ROS-HMGB1 translocation axis is important in the pathogenesis of allergic rhinitis Article 10.1042/CS20201212 1 CLINICAL SCIENCE, v.135, no.3, pp.483 - 494 CLINICAL SCIENCE 135 3 483 494 scie scopus 000615903600004 2-s2.0-85101496390 Medicine, Research & Experimental Research & Experimental Medicine Article GROUP BOX 1 MOBILITY GROUP BOX-1 OXIDATIVE STRESS CONTRIBUTES NEUTROPHILIC ASTHMA AIRWAY INFLAMMATION EPITHELIAL-CELLS HMGB1 PROTEIN CHILDREN BIOMARKER