Ryu, Da Hye Cho, Jwa Yeong Bin Sadiq, Nooruddin Kim, Jin-Chul Lee, Bokyung Hamayun, Muhammad Lee, Taek Sung Kim, Hyoung Seok Park, Soo Hyun Nho, Chu Won Kim, Ho-Youn 2024-01-19T15:33:00Z 2024-01-19T15:33:00Z 2021-09-02 2021-01 0308-8146 https://pubs.kist.re.kr/handle/201004/117520 The dried Ganoderma lucidum (GL) has been widely used for its pharmacological properties and bioactive ganoderic acids (GAs). Herein, extraction procedures combining ultra-sonication and heating were optimized using response surface methodology based on four variables (antioxidant activity, anti-diabetic activity, total GAs content, and total polysaccharide content) and principal component analysis. The extraction of freeze-dried GL at temperatures between 64.2 and 70 degrees C for 1.2 h maximized the antioxidant activity and GA content, whereas the polysaccharide content and anti-diabetic activity were maximized by extraction between 66.8 and 70 degrees C for more than 2.8 h. Heat-dried GL extracted at 50 degrees C for 3 h provided the greatest anti-inflammatory activity against HaCaT cells by suppressing the response to inflammation related cytokines at mRNA levels. These results suggest that extraction conditions might be a limiting factor for target-oriented investigations, and optimized extraction methods may improve the potential effect and quality of harvested GL products. English ELSEVIER SCI LTD Optimization of antioxidant, anti-diabetic, and anti-inflammatory activities and ganoderic acid content of differentially dried Ganoderma lucidum using response surface methodology Article 10.1016/j.foodchem.2020.127645 1 FOOD CHEMISTRY, v.335 FOOD CHEMISTRY 335 N scie scopus 000571176900019 2-s2.0-85089072827 Chemistry, Applied Food Science & Technology Nutrition & Dietetics Chemistry Food Science & Technology Nutrition & Dietetics Article OXIDATIVE STRESS TNF-ALPHA TRITERPENOIDS POLYSACCHARIDES CYTOTOXICITY INFLAMMATION EXPRESSION DISEASE GROWTH CANCER Ganoderma lucidum Ganoderic acid Antioxidant Anti-diabetic Anti-inflammatory Ultrasonic assisted extraction Principal component analysis Response surface methodology