Shin, Sungho Lee, Jeongmin Kwon, Yumi Park, Kang-Sik Jeong, Jae-Hoon Choi, Suk-Joo Bang, Sa Ik Chang, Jong Wook Lee, Cheolju 2024-01-19T15:33:57Z 2024-01-19T15:33:57Z 2022-01-10 2021-01 1661-6596 https://pubs.kist.re.kr/handle/201004/117576 Mesenchymal stem cells (MSCs) have the potential to be a viable therapy against various diseases due to their paracrine effects, such as secretion of immunomodulatory, trophic and protective factors. These cells are known to be distributed within various organs and tissues. Although they possess the same characteristics, MSCs from different sources are believed to have different secretion potentials and patterns, which may influence their therapeutic effects in disease environments. We characterized the protein secretome of adipose (AD), bone marrow (BM), placenta (PL), and Wharton's jelly (WJ)-derived human MSCs by using conditioned media and analyzing the secretome by mass spectrometry and follow-up bioinformatics. Each MSC secretome profile had distinct characteristics depending on the source. However, the functional analyses of the secretome from different sources showed that they share similar characteristics, such as cell migration and negative regulation of programmed cell death, even though differences in the composition of the secretome exist. This study shows that the secretome of fetal-derived MSCs, such as PL and WJ, had a more diverse composition than that of AD and BM-derived MSCs, and it was assumed that their therapeutic potential was greater because of these properties. English MDPI Comparative Proteomic Analysis of the Mesenchymal Stem Cells Secretome from Adipose, Bone Marrow, Placenta and Wharton's Jelly Article 10.3390/ijms22020845 1 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.22, no.2 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22 2 scie scopus 000611312700001 2-s2.0-85099549675 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Article mesenchymal stem cells secretome adipose bone marrow placenta Wharton&#8217 s jelly mass spectrometry