Jung, Hyun-Gug Kim, Ajung Kim, Seung-Chan Park, Jae-Yong Hwang, Eun Mi 2024-01-19T15:34:31Z 2024-01-19T15:34:31Z 2021-09-02 2021-01 2076-3425 https://pubs.kist.re.kr/handle/201004/117611 AEG-1, also called MTDH, has oncogenic potential in numerous cancers and is considered a multifunctional modulator because of its involvement in developmental processes and inflammatory and degenerative brain diseases. However, the role of AEG-1 in astrocytes remains unknown. This study aimed to investigate proteins directly regulated by AEG-1 by analyzing their RNA expression patterns in astrocytes transfected with scramble shRNA and AEG-1 shRNA. AEG-1 knockdown down-regulated TWIK-1 mRNA. Real-time quantitative PCR (qPCR) and immunocytochemistry assays confirmed that AEG-1 modulates TWIK-1 mRNA and protein expression. Electrophysiological experiments further revealed that AEG-1 further regulates TWIK-1-mediated potassium currents in normal astrocytes. An RNA immunoprecipitation assay to determine how AEG-1 regulates the expression of TWIK-1 revealed that AEG-1 binds directly to TWIK-1 mRNA. Furthermore, TWIK-1 mRNA stability was significantly increased upon overexpression of AEG-1 in cultured astrocytes (p < 0.01). Our findings show that AEG-1 serves as an RNA-binding protein to regulate TWIK-1 expression in normal astrocytes. English MDPI AEG-1 Regulates TWIK-1 Expression as an RNA-Binding Protein in Astrocytes Article 10.3390/brainsci11010085 1 BRAIN SCIENCES, v.11, no.1, pp.1 - 11 BRAIN SCIENCES 11 1 1 11 scie scopus 000609830600001 2-s2.0-85099358126 Neurosciences Neurosciences & Neurology Article ELEVATED GENE-1 UP-REGULATION PROMOTES NEURONS CONTRIBUTES PROGRESSION INVASION PATHWAY CATENIN CANCER astrocyte AEG-1 TWIK-1 RNA-binding protein