Moschetta, Matteo Lee, Jong-Young Rodrigues, Joao Podesta, Alice Varvicchio, Omar Son, Jangyup Yangjin Lee Kim, Kwanpyo Lee, Gwan-Hyoung Benfenati, Fabio Bramini, Mattia Capasso, Andrea 2024-01-19T16:00:18Z 2024-01-19T16:00:18Z 2021-09-02 2021-01 2701-0198 https://pubs.kist.re.kr/handle/201004/117643 Graphene is regarded as a viable bio-interface for neuroscience due to its biocompatibility and electrical conductivity, which would contribute to efficient neuronal network signaling. Here, monolayer graphene grown via chemical vapor deposition is treated with remote hydrogen plasma to demonstrate that hydrogenated graphene (HGr) fosters improved cell-to-cell communication with respect to pristine graphene in primary cortical neurons. When transferred to polyethylene terephthalate, HGr exhibits higher wettability than graphene (water contact angle of 83.7 degrees vs 40.7 degrees), while preserving electrical conductivity (approximate to 3 k Omega square (-1)). A rich and mature network is observed to develop onto HGr. The intrinsic excitability and firing properties of neurons plated onto HGr appears unaltered, while the basic passive and active membrane properties are fully preserved. The formation of excitatory synaptic connections increases in HGr with respect to pristine graphene, leading to a doubled miniature excitatory postsynaptic current frequency. This study supports the use of hydrogenation for tailoring graphene into an improved neuronal interface, indicating that wettability, more than electrical conductivity, is the key parameter to be controlled. The use of HGr can bring about a deeper understanding of neuronal behavior on artificial bio-interfaces and provide new insight for graphene-based biomedical applications. English WILEY-V C H VERLAG GMBH Hydrogenated Graphene Improves Neuronal Network Maturation and Excitatory Transmission Article 10.1002/adbi.202000177 1 ADVANCED BIOLOGY, v.5, no.1 ADVANCED BIOLOGY 5 1 N scie scopus 000655685400005 2-s2.0-85108964552 Materials Science, Biomaterials Materials Science Article CHEMICAL-VAPOR-DEPOSITION CULTURED HIPPOCAMPAL-NEURONS CHALLENGES DIFFERENTIATION BEHAVIOR CELLS WATER 2D materials hydrogenation hydrophilicity neurons poly(ethylene terephthalate)