Yanai, Shogo Baba, Takashi Inui, Kai Miyabayashi, Kanako Han, Soyun Inoue, Miki Takahashi, Fumiya Kanai, Yoshiakira Ohkawa, Yasuyuki Choi, Man Ho Morohashi, Ken-ichirou 2024-01-19T16:00:25Z 2024-01-19T16:00:25Z 2021-09-02 2021-01 2045-2322 https://pubs.kist.re.kr/handle/201004/117650 The SRY gene induces testis development even in XX individuals. However, XX/Sry testes fail to produce mature sperm, due to the absence of Y chromosome carrying genes essential for spermatogenesis. XX/Sry Sertoli cells show abnormalities in the production of lactate and cholesterol required for germ cell development. Leydig cells are essential for male functions through testosterone production. However, whether XX/Sry adult Leydig cells (XX/Sry ALCs) function normally remains unclear. In this study, the transcriptomes from XY and XX/Sry ALCs demonstrated that immediate early and cholesterogenic gene expressions differed between these cells. Interestingly, cholesterogenic genes were upregulated in XX/Sry ALCs, although downregulated in XX/Sry Sertoli cells. Among the steroidogenic enzymes, CYP17A1 mediates steroid 17 alpha-hydroxylation and 17,20-lyase reaction, necessary for testosterone production. In XX/Sry ALCs, the latter reaction was selectively decreased. The defects in XX/Sry ALCs, together with those in the germ and Sertoli cells, might explain the infertility of XX/Sry testes. English Nature Publishing Group Gene expression and functional abnormalities in XX/Sry Leydig cells Article 10.1038/s41598-020-80741-z 1 Scientific Reports, v.11, no.1 Scientific Reports 11 1 Y scie scopus 000621920400067 2-s2.0-85099376026 Multidisciplinary Sciences Science & Technology - Other Topics Article STEROIDOGENIC FACTOR-I 3-BETA-HYDROXYSTEROID DEHYDROGENASE TRANSCRIPTIONAL ACTIVATION 17,20-LYASE ACTIVITY CYTOCHROME P450C17 LYASE ACTIVITY TESTIS PROTEIN CHOLESTEROL PHOSPHORYLATION Leydig cell Steroidogenesis SRY gene Sertoli cell CYP17A1