Baek, Su Cheol Yi, Sang Ah Lee, Bum Soo Yu, Jae Sik Kim, Jin-Chul Pang, Changhyun Jang, Tae Su Lee, Jaecheol Kim, Ki Hyun 2024-01-19T16:00:35Z 2024-01-19T16:00:35Z 2021-08-31 2021-01 2227-9059 https://pubs.kist.re.kr/handle/201004/117660 Safflower (Carthamus tinctorius) is an annual herb belonging to the Compositae family; it has a history of use as a food colorant, dye, and medicine in oriental countries. LC-MS-UV-based chemical analysis of extract of the florets of C. tinctorius led to the isolation of two new C-10-polyacetylene glycosides, (8Z)-decaene-4,6-diyne-1,10-diol-1-O-beta-d-glucopyranoside (1) and (8S)-deca-4,6-diyne-1,8-diol-1-O-beta-d-glucopyranoside (2), together with five known analogs (3-7). The structures of the new compounds were determined by using 1D and 2D NMR spectroscopic data and HR-MS data, as well as chemical transformations. Of compounds 1-7, compounds 2, 3, and 4 inhibited the adipogenesis of 3T3-L1 preadipocytes, whereas compounds 1 and 6 promoted adipogenesis. Compounds 2, 3, and 4 also prevented lipid accumulation through the suppression of the expression of lipogenic genes and the increase of the expression of lipolytic genes. Moreover, compounds 3 and 4 activated AMPK, which is known to facilitate lipid metabolism. Our findings provide a mechanistic rationale for the use of safflower-derived polyacetylene glycosides as potential therapeutic agents against obesity. English MDPI Anti-Adipogenic Polyacetylene Glycosides from the Florets of Safflower (Carthamus tinctorius) Article 10.3390/biomedicines9010091 1 BIOMEDICINES, v.9, no.1, pp.1 - 12 BIOMEDICINES 9 1 1 12 scie scopus 000609857100001 2-s2.0-85099831247 Biochemistry & Molecular Biology Medicine, Research & Experimental Pharmacology & Pharmacy Biochemistry & Molecular Biology Research & Experimental Medicine Pharmacology & Pharmacy Article safflower Carthamus tinctorius polyacetylene glycosides AMPK