Shin, Cheol-Hee Byun, Junhyoung Lee, Kijeong Kim, Byoungjae Noh, Yong Kwan Tran, Na Ly Park, Kwideok Kim, Sang-Heon Kim, Tae Hoon Oh, Seung Ja 2024-01-19T16:01:16Z 2024-01-19T16:01:16Z 2021-09-02 2020-12-01 0022-1767 https://pubs.kist.re.kr/handle/201004/117700 Air pollution exposure leads to various inflammatory diseases in the human respiratory system. Chronic rhinosinusitis is an inflammatory disease caused by viruses, bacteria, or air pollutants. However, the underlying molecular mechanisms through which air particulate matter (PM) causes inflammation and disease remain unclear. In this article, we report that the induction of exosomal microRNAs (miRNAs) from human nasal epithelial cells upon airborne PM exposure promotes proinflammatory M1 macrophage polarization via downregulated RORa expression. Exposure of human nasal epithelial cells to PM results in inflammation-related miRNA expression, and more miRNA is secreted through exosomes delivered to macrophages. Among these, miRNA-19a and miRNA-614 directly bind to the 3'-untranslated region of RORa mRNA and downregulate RORa expression, which leads to inflammation due to inflammatory cytokine upregulation and induces macrophages to a proinflammatory M1-like state. Finally, we showed enhanced expression of miRNA-19a and miRNA-614 but reduced RORa expression in a chronic rhinosinusitis patient tissue compared with the normal. Altogether, our results suggest that PM-induced exosomal miRNAs might play a crucial role in the proinflammatory mucosal microenvironment and macrophage polarization through the regulation of RORa expression. English AMER ASSOC IMMUNOLOGISTS EMERGENCY-ROOM VISITS IN-VITRO POLLUTION MICRORNAS HEALTH RESPONSES DIFFERENTIATION RECOGNITION MECHANISM PULMONARY Exosomal miRNA-19a and miRNA-614 Induced by Air Pollutants Promote Proinflammatory M1 Macrophage Polarization via Regulation of ROR alpha Expression in Human Respiratory Mucosal Microenvironment Article 10.4049/jimmunol.2000456 1 JOURNAL OF IMMUNOLOGY, v.205, no.11, pp.3179 - + JOURNAL OF IMMUNOLOGY 205 11 3179 + scie scopus 000593103400022 2-s2.0-85096815799 Immunology Immunology Article EMERGENCY-ROOM VISITS IN-VITRO POLLUTION MICRORNAS HEALTH RESPONSES DIFFERENTIATION RECOGNITION MECHANISM PULMONARY