KIM SI HYUN LIM NU RI Min, Ho phil Sung, Chang min Oh, Han Bin Kim, Ki Hun 2024-01-19T16:01:40Z 2024-01-19T16:01:40Z 2022-01-10 2020-12 2233-4203 https://pubs.kist.re.kr/handle/201004/117723 An analytical method was developed for hypoxia-inducible factor (HIF) stabilizers based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) sample preparation and liquid chromatography？high resolution mass spectrometry analysis. HIF stabilizers potentially enhance the performance of athletes, and hence, they have been prohibited. However, the analysis of urinary HIF stabilizers is not easy owing to their unique structure and characteristics. Hence, we developed the QuEChERS preparation technique for a complementary method and optimized the pH, volume of extraction solvent, and number of extractions. We found that double extraction with 1% of formic acid in acetonitrile provided the highest recovery of HIF stabilizers. Moreover, the composi-tion of the mobile phase was also optimized for better separation of molidustat and IOX4. The developed method was validated in terms of its precision, detection limit, matrix effect, and recovery for ISO accreditation. To the best of our knowledge, this is the first demonstration of the application of the QuEChERS method, which is suitable as a complementary analytical method, in antidoping. ？ 2020, Korean Society for Mass Spectrometry. All rights reserved. English 사단법인 한국질량분석학회 Analysis of hypoxia-inducible factor stabilizers by a modified quechers extraction for antidoping analysis Article 10.5478/MSL.2020.11.4.118 1 Mass Spectrometry Letters, v.11, no.4, pp.118 - 124 Mass Spectrometry Letters 11 4 118 124 Y scopus kci 2-s2.0-85101581332 Article Antidoping Hypoxia-inducible factor stabilizer QuEChERS