Choi, Su Min Kim, Younah Lee, Jaeick Kim, Ju-Hyun Lee, Taeho Min, Byung Sun Kim, Jeong Ah Lee, Sangkyu 2024-01-19T16:01:55Z 2024-01-19T16:01:55Z 2021-09-05 2020-12 0049-8254 https://pubs.kist.re.kr/handle/201004/117738 Hydrocoptisonine is a new compound that has been isolated from the rhizomes ofCoptis chinensis, which belongs to the Ranunculaceae family of Chinese medicines. Although studies onC. chinensishave been reported, the metabolic pathway of hydrocoptisonine in human liver microsomes (HLMs) remains unelucidated. We identified 13 metabolites in HLMs, including six Phase I metabolites and seven glucuronide conjugates, using a high-resolution quadrupole-orbitrap mass spectrometer. The major metabolic pathway was theO-demethylation and mono-hydroxylation of hydrocoptisonine in HLMs. Notably, M3 metabolite wasO-demethylated in dioxolane structures (cyclohexa-3,5-diene-1,2-dione), which was mediated by cytochrome P450 1A2. The locations of hydroxylation and hydroxyl-glucuronidation were identified by analyzing the signature fragments generated as a result of tandem mass spectrometry, indicating hydroxylation at an aliphatic chain or aromatic ring. We determined whether the hydroxylation and glucuronidation occurred in an aromatic moiety (M5 and M12) or an aliphatic moiety (M6 and M13), respectively, based on signature fragments of the metabolites. English TAYLOR & FRANCIS LTD Characterization of hydrocoptisonine metabolites in human liver microsomes using a high-resolution quadrupole-orbitrap mass spectrometer Article 10.1080/00498254.2020.1795304 1 XENOBIOTICA, v.50, no.12, pp.1423 - 1433 XENOBIOTICA 50 12 1423 1433 N scie scopus 000550091000001 2-s2.0-85088267368 Pharmacology & Pharmacy Toxicology Pharmacology & Pharmacy Toxicology Article IN-VITRO METABOLITES RAT BERBERINE CYPs hydrocoptisonine human liver microsomes O-demethylation UGT