Choi, Tae-Rim Park, Ye-Lim Song, Hun-Suk Lee, Sun Mi Park, Sol Lee Lee, Hye Soo Kim, Hyun-Joong Bhatia, Shashi Kant Gurav, Ranjit Lee, Yoo Kyung Sung, Changmin Yang, Yung-Hun 2024-01-19T16:02:30Z 2024-01-19T16:02:30Z 2021-09-02 2020-12 1367-5435 https://pubs.kist.re.kr/handle/201004/117775 Psychrophilic bacteria, living at low and mild temperatures, can contribute significantly to our understanding of microbial responses to temperature, markedly occurring in the bacterial membrane. Here, a newly isolated strain, Pseudomonas sp. B14-6, was found to dynamically change its unsaturated fatty acid and cyclic fatty acid content depending on temperature which was revealed by phospholipid fatty acid (PLFA) analysis. Genome sequencing yielded the sequences of the genes Delta-9-fatty acid desaturase (desA) and cyclopropane-fatty acid-acyl-phospholipid synthase (cfa). Overexpression of desA in Escherichia coli led to an increase in the levels of unsaturated fatty acids, resulting in decreased membrane hydrophobicity and increased fluidity. Cfa proteins from different species were all found to promote bacterial growth, despite their sequence diversity. In conclusion, PLFA analysis and genome sequencing unraveled the temperature-related behavior of Pseudomonas sp. B14-6 and the functions of two membrane-related enzymes. Our results shed new light on temperature-dependent microbial behaviors and might allow to predict the consequences of global warming on microbial communities. English SPRINGER HEIDELBERG PALM BIOMASS HYDROLYSATE RESISTANCE PLFA TEMPERATURE PHYLOGENIES FLUIDITY Effects of a Delta-9-fatty acid desaturase and a cyclopropane-fatty acid synthase from the novel psychrophile Pseudomonas sp. B14-6 on bacterial membrane properties Article 10.1007/s10295-020-02333-0 1 JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.47, no.12, pp.1045 - 1057 JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 47 12 1045 1057 scie scopus 000595029100001 2-s2.0-85096988686 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article PALM BIOMASS HYDROLYSATE RESISTANCE PLFA TEMPERATURE PHYLOGENIES FLUIDITY Pseudomonas strain Phospholipid fatty acid Genome sequencing Hydrophobicity Membrane fluidity