Lee, Kyungwoo Min, Daejin Choi, Yonghyun Kim, Jiwon Yoon, Semi Jang, Jaehee Park, Soomin Tanaka, Masayoshi Cho, Yong Woo Koo, Hyung-Jun Jeon, Hojeong Choi, Jonghoon 2024-01-19T16:03:01Z 2024-01-19T16:03:01Z 2021-09-02 2020-12 1860-6768 https://pubs.kist.re.kr/handle/201004/117807 The topical delivery of siRNA-based therapies has opened new avenues for the treatment of skin disorders. The use of siRNA as a therapeutic, however, is limited due to its rapid degradation and poor cellular uptake. Furthermore, the top layer of skin, the stratum corneum, is a major barrier to the delivery of topical agents. There is an unmet need for efficient topical formulations for delivering siRNA to the site of action. In this study, 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) or lipofectamine is used to prepare a nanocarrier for delivering siRNA against glyceraldehyde 3-phosphate dehydrogenase (GAPDH); GAPDH expression is then evaluated at the cellular level. In addition, a dermal transport assay is designed and implemented to evaluate the penetration and delivery efficacy of siRNA in pig skin using lipid nanocarriers. The delivery of siRNA with the use of a lipid nanocarrier is significantly better than the delivery of siRNA without it. Thus, the findings identify lipid nanocarriers as excellent candidates for the transdermal delivery of siRNA for gene silencing in the skin and thus for applications in related preclinical models. English Wiley - VCH Verlag GmbH & CO. KGaA Study and Evaluation of the Potential of Lipid Nanocarriers for Transdermal Delivery of siRNA Article 10.1002/biot.202000079 1 Biotechnology Journal, v.15, no.12 Biotechnology Journal 15 12 N scie scopus 000570179800001 2-s2.0-85091011272 Biochemical Research Methods Biotechnology & Applied Microbiology Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Article BETA-GLUCAN NANOPARTICLES MOLECULES ANTITUMOR PEPTIDES CELLS 1 2-dioleoyl-3-trimethylammonium-propane gene delivery lipofectamine nanocarrier siRNA transdermal