Bong, Ji-Hong Kim, Tae-Hun Jung, Jaeyong Lee, Soo Jeong Sung, Jeong Soo Lee, Chang Kyu Kang, Min-Jung Kim, Hyun Ok Pyun, Jae-Chul 2024-01-19T16:03:06Z 2024-01-19T16:03:06Z 2021-09-02 2020-12 1976-0280 https://pubs.kist.re.kr/handle/201004/117811 Anti-coronavirusdisease-2019 (COVID-19; anti-severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2)) antibodies against nucleoprotein (NP) were purified from pig sera. Following the separation of the antibody fraction using a protein-A column, the final yield of the purified antibodies against SARS-CoV-2 NPs was estimated to be 0.26 +/- 0.05 % (absolute amount of 143.4 +/- 25.2 ng, n=5) from 1 mL of pig sera. The binding activities of the isolated antibodies were confirmed using immunoassay and immunostaining. Based on the specific binding activity to NPs, a quantitative assay was performed using a surface plasmon resonance (SPR) biosensor. From the doseresponse curve, the binding constant (Kd) was calculated to be 185 pM and the limit of detection was estimated to be 1.02 pM. The SPR biosensor with the isolated antibodies against SARS-CoV-2 NPs was applied for the detection of SARS-CoV-2, MERS-CoV, and CoV strain 229E in culture fluid. English KOREAN BIOCHIP SOCIETY-KBCS PROTEIN Pig Sera-derived Anti-SARS-CoV-2 Antibodies in Surface Plasmon Resonance Biosensors Article 10.1007/s13206-020-4404-z 1 BIOCHIP JOURNAL, v.14, no.4, pp.358 - 368 BIOCHIP JOURNAL 14 4 358 368 scie scopus kci 000582763900001 2-s2.0-85094109212 Biochemical Research Methods Chemistry, Analytical Nanoscience & Nanotechnology Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Article PROTEIN SARS-CoV-2 COVID-19 Antibody Nucleoprotein Surface plasmon resonance Immunoassay