Yoo, Jin Park, Ji Hun Kwon, Young Woo Chung, Justin J. Choi, In Cheul Nam, Jae Joon Lee, Hyun Su Jeon, Eun Young Lee, Kangwon Kim, Soo Hyun Jung, Youngmee Park, Jong Woong 2024-01-19T16:03:29Z 2024-01-19T16:03:29Z 2021-09-02 2020-11-21 2047-4830 https://pubs.kist.re.kr/handle/201004/117835 Peripheral nerve injury results in significant sensory and motor functional deficits. Although direct neurorrhaphy in the early phase may reduce its devastating effects, direct end-to-end neurorrhaphy is sometimes impossible owing to a defect at the injured site of the nerve. Autogenous nerve graft is a primary consideration for peripheral nerve defects; however, significant morbidity of the donor site is inevitable. Recently, the treatment using engineered synthetic nerve conduits has been regarded as a promising strategy to promote the regeneration of peripheral nerve defects. In this study, we developed longitudinally oriented collagen hydrogel-grafted elastic nerve guidance conduits (NGC) to reconstruct sciatic nerve defects. An elastic NGC was prepared by using poly(lactide-co-caprolactone) (PLCL), and electrospun PLCL was adopted to fabricate nanoporous structures with appropriate permeability for nerve regeneration. Oriented collagen hydrogels were prepared by the 3D printing method to achieve a microscale hydrogel pattern. Based on sciatic nerve injury models in rats, we confirmed the beneficial effects of the NGC with 3D printed collagen hydrogel on axonal regeneration and remyelination along with superior functional recovery in comparison with the NGC filled with the bulk collagen hydrogel. It is believed that the aligned collagen hydrogels provide a preferable environment for nerve regeneration, functioning as an oriented guidance path. In conclusion, the PLCL nerve guide conduit containing a 3D printed aligned collagen hydrogel can be useful for peripheral nerve regeneration. English ROYAL SOC CHEMISTRY GROWTH-FACTOR SCAFFOLDS CHITOSAN MATRICES BEHAVIOR MODEL Augmented peripheral nerve regeneration through elastic nerve guidance conduits prepared using a porous PLCL membrane with a 3D printed collagen hydrogel Article 10.1039/d0bm00847h 1 BIOMATERIALS SCIENCE, v.8, no.22, pp.6261 - 6271 BIOMATERIALS SCIENCE 8 22 6261 6271 scie scopus 000588260700007 2-s2.0-85096080237 Materials Science, Biomaterials Materials Science Article GROWTH-FACTOR SCAFFOLDS CHITOSAN MATRICES BEHAVIOR MODEL