Lee, S.R. Choi, J.H. Ryoo, R. Kim, J.-C. Pang, C. Kim, S.-H. Kim, K.H. 2024-01-19T16:04:19Z 2024-01-19T16:04:19Z 2021-09-02 2020-11 1017-7825 https://pubs.kist.re.kr/handle/201004/117884 As part of our current work to discover structurally and/or biologically novel compounds from Korean wild mushrooms, we isolated five ergostane-type steroids (1-5) from the fruiting bodies of Xerula furfuracea via repeated column chromatographic separations and HPLC purification. The chemical structures of the isolated steroids were shown to be (22E,24R)-24-methylcholesta-4,22-diene-3,6-dione (1), ergosta-7,22-diene-3β,5α,6β-triol (2), ergosta-7,22-diene-3β,5α,6β,9α-tetraol (3), (22E,24R)-5α,8α-epidioxyergosta-6,22-diene-3β-ol-3-O-β-D-glucopyranoside (4), and (22E,24R)-5α,8α-epidioxyergosta-6,9,22-triene-3β-ol-3-O-β-D-glucopyranoside (5) based on comparison of the data regarding their spectroscopic and physical properties with those of previous studies. Notably, this is the first report on the presence of the identified steroids (1-5) in this mushroom. We tested compounds 1？5 to determine their effects on adipogenesis and osteogenesis in the mouse mesenchymal stem cell line C3H10T1/2 and found that compounds 4 and 5 suppressed the differentiation of stem cells into adipocytes. Notably, in addition to its suppressive effect on adipogenesis, compound 5 was also shown to promote the osteogenic differentiation of stem cells. These findings demonstrate that the bioactive compounds isolated might be effective for the treatment of menopause-associated syndromes, such as osteoporosis and obesity, as the isolated compounds were shown to suppress adipogenesis and/or promote osteogenesis of stem cells. ？ 2020 Korean Society for Microbiology and Biotechnology. All rights reserved. English Korean Society for Microbiolog and Biotechnology 24 methylcholesta 4,22 diene 3,6 dione 5alpha,8alpha epidioxyergosta 6,22 diene 3betaol 3o beta dextro glucopyranoside 5alpha,8alpha epidioxyergosta 6,9,22 triene 3beta ol 3 o beta dextro glucopyranoside ergosta 7,22 diene 3beta,5alpha,6beta triol ergosta 7,22 diene 3beta,5alpha,6beta,9alpha tetraol plant extract steroid unclassified drug Xerula furfuracea extract adipocyte adipogenesis Agaricales animal cell Article bone development carbon nuclear magnetic resonance cell differentiation column chromatography controlled study drug identification drug isolation drug structure embryo high performance liquid chromatography mesenchymal stem cell mouse nonhuman osteoblast proton nuclear magnetic resonance real time polymerase chain reaction traditional medicine Xerula furfuracea Ergostane-type steroids from Korean wild mushroom xerula furfuracea that control adipocyte and osteoblast differentiation Article 10.4014/JMB.2006.06013 1 Journal of Microbiology and Biotechnology, v.30, no.11, pp.1769 - 1776 Journal of Microbiology and Biotechnology 30 11 1769 1776 scie scopus kci ART002649804 000595945500005 2-s2.0-85097003654 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article traditional medicine Xerula furfuracea 24 methylcholesta 4,22 diene 3,6 dione 5alpha,8alpha epidioxyergosta 6,22 diene 3betaol 3o beta dextro glucopyranoside 5alpha,8alpha epidioxyergosta 6,9,22 triene 3beta ol 3 o beta dextro glucopyranoside ergosta 7,22 diene 3beta,5alpha,6beta triol ergosta 7,22 diene 3beta,5alpha,6beta,9alpha tetraol plant extract steroid unclassified drug Xerula furfuracea extract adipocyte adipogenesis Agaricales animal cell Article bone development carbon nuclear magnetic resonance cell differentiation column chromatography controlled study drug identification drug isolation drug structure embryo high performance liquid chromatography mesenchymal stem cell mouse nonhuman osteoblast proton nuclear magnetic resonance real time polymerase chain reaction Adipogenesis Ergostane-type steroids Osteogenesis Physalacriaceae Xerula furfuracea