Lee, Kyungwoo Min, Daejin Choi, Yonghyun Yoon, Semi Jang, Jaehee Hwang, Jangsun Jeon, Hojeong Cho, Yong Woo Choi, Jonghoon 2024-01-19T16:04:33Z 2024-01-19T16:04:33Z 2021-09-02 2020-11 https://pubs.kist.re.kr/handle/201004/117898 We aimed to design and manufacture a transporter capable of delivering small interfering RNAs (siRNAs) into the skin without causing any damage. beta-glucans are unique chiral polysaccharides with well-defined immunological properties and supramolecular wrapping ability. However, the chiral properties of these polymers have hardly been applied in drug delivery systems. In this study, beta-glucan nanoparticles were designed and manufactured to deliver genetic material to the target cells. The beta-glucan molecules were self-assembled with an siRNA into nanoparticles of 300-400 nm in diameter via a conformational transition process, in order to construct a gene delivery system. The assembled gene nanocarriers were associated with high gene-loading ability. The expression and efficiency of siRNA were verified after its delivery via beta-glucan. Our results provide evidence that beta-glucan nanoparticles can be effectively used to deliver siRNA into the cells. English MDPI AG Self-Assembling β-Glucan Nanomedicine for the Delivery of siRNA Article 10.3390/biomedicines8110497 1 Biomedicines, v.8, no.11 Biomedicines 8 11 Y scie scopus 000593501200001 2-s2.0-85096046717 Biochemistry & Molecular Biology Medicine, Research & Experimental Pharmacology & Pharmacy Biochemistry & Molecular Biology Research & Experimental Medicine Pharmacology & Pharmacy Article NANOPARTICLES ANTITUMOR PEPTIDES CELLS self-assembly nanomedicine siRNA gene delivery beta-glucan