Park, Sang-a Bong, Sim-Kyu Lee, Jin Woo Park, No-June Choi, Yongsoo Kim, Sang Moo Yang, Min Hye Kim, Yong Kee Kim, Su-Nam 2024-01-19T16:04:36Z 2024-01-19T16:04:36Z 2021-09-02 2020-11 1976-9148 https://pubs.kist.re.kr/handle/201004/117900 Naturally derived diosmetin and its glycoside diosmin are known to be effective in treating inflammatory disease. This study was performed to determine whether diosmin and diosmetin have the effect of improving atopic dermatitis in a 2,4-dinitrochlorobenzen (DNCB)-induced atopic dermatitis (AD) model. DNCB was used to establish AD model in hairless mice. Skin moisture, serum immunoglobulin E (IgE), interleukin 4 (IL-4), and histological analysis were performed to measure the effectiveness of diosmin and diosmetine to improve AD. IL-4 levels were also measured in RBL-2H3 cells. Administration of diosmetin or diosmin orally inhibited the progress of DNCB-induced AD-like lesions in murine models by inhibiting transdermal water loss (TEWL) and increasing skin hydration. Diosmetin or diosmin treatment also reduced IgE and IL-4 levels in AD-induced hairless mouse serum samples. However, in the in vitro assay, only diosmetin, not diosmin, reduced the expression level of IL-4 mRNA in RBL-2H3 cells. Diosmin and diosmetine alleviated the altered epidermal thickness and immune cell infiltration in AD. Diosmin is considered effective in the cure of AD and skin inflammatory diseases by being converted into diosmetin in the body by pharmacokinetic metabolism. Thus, oral administration of diosmetin and diosmin might be a useful agent for the treatment of AD and cutaneous inflammatory diseases. English KOREAN SOC APPLIED PHARMACOLOGY OXIDATIVE STRESS FLAVONOIDS CARCINOGENESIS MODULATION MECHANISM Diosmetin and Its Glycoside, Diosmin, Improve Atopic Dermatitis-Like Lesions in 2,4-Dinitrochlorobenzene-Induced Murine Models Article 10.4062/biomolther.2020.135 1 BIOMOLECULES & THERAPEUTICS, v.28, no.6, pp.542 - 548 BIOMOLECULES & THERAPEUTICS 28 6 542 548 scie scopus kci ART002638305 000614912400007 2-s2.0-85094123345 Biochemistry & Molecular Biology Pharmacology & Pharmacy Biochemistry & Molecular Biology Pharmacology & Pharmacy Article OXIDATIVE STRESS FLAVONOIDS CARCINOGENESIS MODULATION MECHANISM Diosmetin Diosmin Atopic dermatitis IL-4 DNCB