Shin, Joonchul Song, Young Geun Jung, Sung-Jin Yoon, Taeehee Kim, Gwang Su Kim, Jeong Hun Park, Hyung-Ho Ju, Byeong-Kwon Kim, Seong Keun Baek, Seung-Hyub Jung, Hyo-Il Kang, Chong-Yun Kim, Jin-Sang 2024-01-19T16:30:53Z 2024-01-19T16:30:53Z 2021-09-02 2020-11 0925-4005 https://pubs.kist.re.kr/handle/201004/117966 Microbial forensics, exploiting bacteria, archaea, and eukaryotes, has been considered as one of the primary fields to trace the postmortem interval from the decaying cadavers. On the other hand, there remain several challenges of laboratory-based analysis for prediction of postmortem interval, including long-time measurement, complicated measuring procedure, and bacterial growth while carrying samples from the scene. Herein, we introduce the Smart Forensic Kit, which consists of a highly sensitive colorimetric gas sensor, a quality control algorithm, and a smartphone-based analysis method, to quantify the bacterial-derived ammonia gas in real-time. As a result, the estimation system of the postmortem interval has a superior selectivity to the ammonia gas with a detection limit of 38.7 ppb, response linearity to the target bacteria (Escherichia colt , Pseudomonas aeruginosa, and Pseudomonas putida), and short measuring time (10 min) with the maximum predicted postmortem interval from the mouse carcass (168 h). Furthermore, thanks to measuring the postmortem interval within 10 min, the negligible increase rate of bacterial concentration was observed. Consequently, the results reflected a high correlation between the ammonia gas emitted from bacteria and the postmortem interval so that we believe the Smart Forensic Kit will be applied for tracing down the decomposition of the cadavers in the near future. English ELSEVIER SCIENCE SA Smart forensic kit: Real-time estimation of postmortem interval using a highly sensitive gas sensor for microbial forensics Article 10.1016/j.snb.2020.128612 1 SENSORS AND ACTUATORS B-CHEMICAL, v.322 SENSORS AND ACTUATORS B-CHEMICAL 322 N scie scopus 000573297000004 2-s2.0-85088866614 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article AMMONIA PRODUCTION AMINES LEVEL STRIP Postmortem interval Colorimetric analysis Polymer-based gas sensor Bromophenol blue Bacteria