Kim, Yun Ah Chun, So Young Park, Sung-Bin Kang, Eunyoung Koh, Won-Gun Kwon, Tae Gyun Han, Dong Keun Joung, Yoon Ki 2024-01-19T16:31:30Z 2024-01-19T16:31:30Z 2021-09-02 2020-10-07 2047-4830 https://pubs.kist.re.kr/handle/201004/118003 Fibroblast-derived extracellular matrix (fECM)-supported scaffolds made up of poly(lactic-co-glycolic acid) were prepared with the enhanced preservation of ECM components by composites with magnesium hydroxide nanoparticles (MH NPs), and were applied for the renal tissue regeneration. MH NP utilization resulted in an increased ECM protein amount, decreased scaffold degradation, and surface hydrophilic modification. These effects were correlated with the improved adhesion and viability of renal proximal tubule epithelial cells on the scaffold.In vivoexperiments demonstrated effects of fECM and MH NPs on renal regeneration. The number of glomeruli was the largest in the ECM scaffold with MH NPs as compared to the pristine scaffold and ECM scaffold without MH NPs. Quantitative PCR analysis exhibited less inflammation (IL-1 beta, TNF-alpha, and IL-6) and fibrosis-related (vimentin, collagen I, and alpha-SMA) markers, whereas opposite results were found in regeneration-related markers (Pax2, vWf, Wt1, and Emx2). The concentration of renal function-related molecules, creatinine and blood urea nitrogen diminished in the ECM scaffold with MH NPs. All results indicate that MH NPs utilization for the renal regenerative scaffold is effective forin vitroandin vivoenvironments and is, therefore, a good model for regeneration of kidneys and other tissues, and organs. English ROYAL SOC CHEMISTRY CELL-ADHESION KIDNEY-DISEASE STEM-CELLS PROLIFERATION STRATEGIES STIFFNESS DEVICE OXIDE PLGA Scaffold-supported extracellular matrices preserved by magnesium hydroxide nanoparticles for renal tissue regeneration Article 10.1039/d0bm00871k 1 BIOMATERIALS SCIENCE, v.8, no.19, pp.5427 - 5440 BIOMATERIALS SCIENCE 8 19 5427 5440 scie scopus 000573820100012 2-s2.0-85092681869 Materials Science, Biomaterials Materials Science Article CELL-ADHESION KIDNEY-DISEASE STEM-CELLS PROLIFERATION STRATEGIES STIFFNESS DEVICE OXIDE PLGA