Han, Bo Hoon Kim, Sumi Seo, Geeyoon Heo, Youhee Chung, Seok Kang, Ji Yoon 2024-01-19T16:32:43Z 2024-01-19T16:32:43Z 2021-09-02 2020-10 1473-0197 https://pubs.kist.re.kr/handle/201004/118077 As conventional bulky methods for extracellular vesicle (EV) separation are unsuitable for small volumes of samples, microfluidic devices are thought to offer a solution for the integrated and automatic processing of EV separation. This study demonstrates a simple microfluidic aqueous two-phase system (ATPS) for EV separation with high recovery efficiency to overcome the limitation of previous devices, which require complex external equipment or high cost manufacturing. With polyethylene glycol and dextran in the microfluidic channel, the isolation mechanism of the microfluidic ATPS was analyzed by comparison between two-phase and one-phase systems. Our device could facilitate continuous EV isolation with 83.4% recovery efficiency and remove 65.4% of the proteins from the EV-protein mixture. EVs were also successfully isolated from human plasma at high recovery efficiency. English Royal Society of Chemistry Isolation of extracellular vesicles from small volumes of plasma using a microfluidic aqueous two-phase system Article 10.1039/d0lc00345j 1 Lab on a Chip, v.20, no.19, pp.3552 - 3559 Lab on a Chip 20 19 3552 3559 N scie scopus 000573314400003 2-s2.0-85092219847 Biochemical Research Methods Chemistry, Multidisciplinary Chemistry, Analytical Nanoscience & Nanotechnology Instruments & Instrumentation Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Instruments & Instrumentation Article EXTRACTION SEPARATION HYDROPHOBICITY BIOMARKERS WATER LATERAL DISPLACEMENT ARRAYS CELL-CULTURE SUPERNATANT EXOSOME ISOLATION PARTITIONING BEHAVIOR POLYETHYLENE-GLYCOL