Lee, Seulah Lee, Dahae Ryoo, Rhim Kim, Jin-Chul Park, Hyun Bong Kang, Ki Sung Kim, Ki Hyun 2024-01-19T16:33:07Z 2024-01-19T16:33:07Z 2021-08-31 2020-09-25 0163-3864 https://pubs.kist.re.kr/handle/201004/118101 Calvatia nipponica is an extremely rare mushroom with a limited number of studies on its chemical components and biological activities published. Here we report the isolation of a novel sterol, calvatianone (1), possessing a 6/5/6/5-fused ring system with a contracted tetrahydrofuran B-ring, and four known steroids (2-5) from the fruiting bodies of C. nipponica. The structure of calvatianone including its absolute configuration was determined by NMR spectroscopic analyses, HR-ESIMS, gauge-including atomic orbital NMR chemical shift calculations, and ECD calculations. Ergosterol peroxide (3) and cyathisterol (4) suppressed the cell viability increase induced by 17 beta-estradiol in MCF-7 breast cancer cell lines, suggesting a possible approach for these compounds to serve as ER alpha antagonists. English AMER CHEMICAL SOC BIOACTIVITY-GUIDED ISOLATION LYCOPERDACEAE CONSTITUENTS FUNGUS Calvatianone, a Sterol Possessing a 6/5/6/5-Fused Ring System with a Contracted Tetrahydrofuran B-Ring, from the Fruiting Bodies of Calvatia nipponica Article 10.1021/acs.jnatprod.0c00673 1 JOURNAL OF NATURAL PRODUCTS, v.83, no.9, pp.2737 - 2742 JOURNAL OF NATURAL PRODUCTS 83 9 2737 2742 scie scopus 000575848000022 2-s2.0-85091653906 Plant Sciences Chemistry, Medicinal Pharmacology & Pharmacy Plant Sciences Pharmacology & Pharmacy Article BIOACTIVITY-GUIDED ISOLATION LYCOPERDACEAE CONSTITUENTS FUNGUS