Jeon, Joong-won Choi, Nakwon Lee, Seung Hwan Sung, Jong Hwan 2024-01-19T16:33:33Z 2024-01-19T16:33:33Z 2021-09-02 2020-09-11 1387-2176 https://pubs.kist.re.kr/handle/201004/118126 The interaction between the gut and the liver, often known as the gut-liver axis, play crucial roles in modulating the body's responses to the xenobiotics as well as progression of diseases. Dysfunction of the axis can cause metabolic disorders as well as obesity, diabetes, and fatty liver disease. During the progression of such diseases, inflammatory responses involving the immune system also play an important part. In this study, we developed a three-tissue microphysiological system (MPS) that can accommodate three different cell types in separated compartments connected via fluidic channels in a microfluidic device. Using computational fluid dynamics, geometry of fluidic channels and flow conditions were optimized for seeding and culturing different cell types in the three-tissue MPS. Caco-2 (gut), RAW264.7 (immune), and HepG2 (liver) cells were seeded and cultured in the chip. Stimulation of the gut cells in the MPS with lipopolysaccharide (LPS) resulted in induction of inflammatory response and production of nitric oxide (NO) in all connected chambers. The anti-inflammatory effect of luteolin was demonstrated. Our study demonstrates that the three-tissue MPS can recapitulate the inflammatory responses involving the gut, liver and immune cells. English SPRINGER INCREASED INTESTINAL PERMEABILITY IN-VITRO MODEL NITRIC-OXIDE BACTERIAL OVERGROWTH EPITHELIAL-CELLS IRRITABLE-BOWEL HEPG2 CELLS LEAKY GUT MICROBIOTA PATHOGENESIS Three-tissue microphysiological system for studying inflammatory responses in gut-liver Axis Article 10.1007/s10544-020-00519-y 1 BIOMEDICAL MICRODEVICES, v.22, no.4 BIOMEDICAL MICRODEVICES 22 4 scie scopus 000568306400001 2-s2.0-85090503724 Engineering, Biomedical Nanoscience & Nanotechnology Engineering Science & Technology - Other Topics Article INCREASED INTESTINAL PERMEABILITY IN-VITRO MODEL NITRIC-OXIDE BACTERIAL OVERGROWTH EPITHELIAL-CELLS IRRITABLE-BOWEL HEPG2 CELLS LEAKY GUT MICROBIOTA PATHOGENESIS Gut-liver axis Microphysiological systems (MPS) Inflammation Organ-on-a-chip