Kim, Hong-Rae Bong, Ji-Hong Jung, Jaeyong Sung, Jeong Soo Kang, Min-Jung Park, Jae-Gwan Pyun, Jae-Chul 2024-01-19T16:34:41Z 2024-01-19T16:34:41Z 2021-09-02 2020-09 1976-0280 https://pubs.kist.re.kr/handle/201004/118192 The passivation layers of anin situ-synthesized cadmium sulfide (CdS) nanowire (NW) photosensor were prepared for two reasons: (1) to improve the physical stability of the NW on an interdigitated electrode and (2) to enhance the immobilization efficiency of proteins for the on-chip immunoassay. The passivation layer was estimated to have suitable optical properties, and the photoresponse of photosensor was increased after the formation of passivation layer. The immobilization efficiency of a parylene-H film through covalent bonding was compared with the immobilization of Z-domains through physical adsorption. Finally, on-chip chemiluminescent immunoassay of bacteria was carried out by immobilizing antibodies againstEscherichia colithrough Z-domains for the orientation control of antibodies. The limit of detection was determined to be less than 10(4)E. coli/mL (n=3), and the sensitivity of bacterial detection was estimated to be 0.339 pA/E. coli/mL (n=3) with a linearity factor (R-2) of 0.999. These results showed that the on-chip chemiluminescent immunoassay for bacterial detection could be performed using passivation layer coated CdS NW photosensor. English KOREAN BIOCHIP SOCIETY-KBCS SURFACE MODIFICATION ESCHERICHIA-COLI OUTER-MEMBRANE PARYLENE-N FILMS An On-chip Chemiluminescent Immunoassay for Bacterial Detection using in Situ-synthesized Cadmium Sulfide Nanowires with Passivation Layers Article 10.1007/s13206-020-4305-1 1 BIOCHIP JOURNAL, v.14, no.3, pp.268 - 278 BIOCHIP JOURNAL 14 3 268 278 scie scopus kci ART002625646 000565121800003 2-s2.0-85090148360 Biochemical Research Methods Chemistry, Analytical Nanoscience & Nanotechnology Biochemistry & Molecular Biology Chemistry Science & Technology - Other Topics Article SURFACE MODIFICATION ESCHERICHIA-COLI OUTER-MEMBRANE PARYLENE-N FILMS CdS nanowire Photosensor Surface passivation On-chip immunoassay