Lee, Dahae Lee, Jin Su Sezirahiga, Jurdas Kwon, Hak Cheol Jang, Dae Sik Kang, Ki Sung 2024-01-19T17:00:16Z 2024-01-19T17:00:16Z 2021-09-02 2020-09 2223-7747 https://pubs.kist.re.kr/handle/201004/118212 Chocolate vine (Akebia quinata) is consumed as a fruit and is also used in traditional medicine. In order to identify the bioactive components ofA. quinata, a phytosterol glucoside stigmasterol-3-O-beta-D-glucoside (1), three triterpenoids maslinic acid (2), scutellaric acid (3), and hederagenin (4), and three triterpenoidal saponins akebia saponin PA (5), hederacoside C (6), and hederacolchiside F (7) were isolated from a 70% EtOH extract of the fruits ofA. quinata(AKQU). The chemical structures of isolates 1-7 were determined by analyzing the 1D and 2D nuclear magnetic resonance (NMR) spectroscopic data. Here, we evaluated the effects of AKQU and compounds 1-7 on insulin secretion using the INS-1 rat pancreatic beta-cell line. Glucose-stimulated insulin secretion (GSIS) was evaluated in INS-1 cells using the GSIS assay. The expression levels of the proteins related to pancreatic beta-cell function were detected by Western blotting. Among the isolates, stigmasterol-3-O-beta-D-glucoside (1) exhibited strong GSIS activity and triggered the overexpression of pancreas/duodenum homeobox protein-1 (PDX-1), which is implicated in the regulation of pancreatic beta-cell survival and function. Moreover, isolate 1 markedly induced the expression of extracellular signal-regulated protein kinases 1 and 2 (ERK1/2), insulin receptor substrate-2 (IRS-2), phosphoinositide 3-kinase (PI3K), and Akt, which regulate the transcription of PDX-1. The results of our experimental studies indicated that stigmasterol-3-O-beta-D-glucoside (1) isolated from the fruits ofA. quinatacan potentially enhance insulin secretion, and might alleviate the reduction in GSIS during the development of T2DM. English MDPI MASLINIC ACID PENTACYCLIC TRITERPENES BIOLOGICAL EVALUATION GENE-EXPRESSION OLIVE FRUIT GLYCOSIDES SAPONINS IDENTIFICATION CONSTITUENTS ACTIVATION Bioactive Phytochemicals Isolated from Akebia quinata Enhances Glucose-Stimulated Insulin Secretion by Inducing PDX-1 Article 10.3390/plants9091087 1 PLANTS-BASEL, v.9, no.9 PLANTS-BASEL 9 9 scie scopus 000580381300001 2-s2.0-85090611286 Plant Sciences Plant Sciences Article MASLINIC ACID PENTACYCLIC TRITERPENES BIOLOGICAL EVALUATION GENE-EXPRESSION OLIVE FRUIT GLYCOSIDES SAPONINS IDENTIFICATION CONSTITUENTS ACTIVATION Akebia quinata stigmasterol-3-O-beta-D-glucoside insulin secretion IRS-2 PI3K Akt PDX-1