Kim, Eun-Nam Kwon, Jaeyoung Lee, Hyun-Su Lee, Sooyeun Lee, Dongho Jeong, Gil-Saeng 2024-01-19T17:01:11Z 2024-01-19T17:01:11Z 2021-09-02 2020-08-05 1663-9812 https://pubs.kist.re.kr/handle/201004/118266 Cudratrixanthone U (CTU) is a prenylated xanthone compound isolated fromMaclura tricuspidataBureau (Moraceae). Prenylated xanthones have been reported to exhibit a variety of biological activities. However, the effects of prenylated xanthone on osteoclast differentiation and function are still unclear. Excessive bone resorption by osteoclasts is considered a major cause of diseases such as osteoporosis. Accordingly, suppression of excessive osteoclast formation and function is one of strategies for treating osteoclast related bone diseases. In this study, CTU inhibited osteoclast differentiation and function in RAW264.7 macrophages and BMM cells induced by receptor activator of nuclear factor-kappa B ligand (RANKL). CTU regulated the formation of TRAF6-TAK1 complex in RANKL-induced RAW264.7 macrophages and BMM cells. Osteoclast-specific genes including those encoding matrix metallopeptidase 9 (MMP-9), dendritic cell-specific transmembrane proteins (DC-STAMP), cathepsin K (CTSK) and chemokine CC motif ligand 4 (CCL4) play an important role in bone resorption and migration, and were effectively regulated by CTU. These results suggest that CTU is a potential therapeutic agent in osteoporosis. English FRONTIERS MEDIA SA NF-KAPPA-B CUDRANIA-TRICUSPIDATA RECEPTOR ACTIVATOR BONE-RESORPTION DOWN-REGULATION ROOT BARK C-FOS ANTIOXIDANT INDUCTION CONSTITUENTS Inhibitory Effect of Cudratrixanthone U on RANKL-Induced Osteoclast Differentiation and Function in Macrophages and BMM Cells Article 10.3389/fphar.2020.01048 1 FRONTIERS IN PHARMACOLOGY, v.11 FRONTIERS IN PHARMACOLOGY 11 scie scopus 000563471600001 2-s2.0-85089807157 Pharmacology & Pharmacy Pharmacology & Pharmacy Article NF-KAPPA-B CUDRANIA-TRICUSPIDATA RECEPTOR ACTIVATOR BONE-RESORPTION DOWN-REGULATION ROOT BARK C-FOS ANTIOXIDANT INDUCTION CONSTITUENTS Cudratrixanthone U TRAF6 TAK-1 RANKL CCL4 osteoclast