Son, Ye Seul Ki, Soo Jin Thanavel, Rajangam Kim, Jong-Jin Lee, Mi-Ok Kim, Janghwan Jung, Cho-Rok Han, Tae-Su Cho, Hyun-Soo Ryu, Choong-Min Kim, Sang-Heon Park, Doo-Sang Son, Mi-Young 2024-01-19T17:01:43Z 2024-01-19T17:01:43Z 2021-09-05 2020-08 0892-6638 https://pubs.kist.re.kr/handle/201004/118293 Lactobacilli, which are probiotic commensal bacteria that mainly reside in the human small intestine, have attracted attention for their ability to exert health-promoting effects and beneficially modulate host immunity. However, host epithelial-commensal bacterial interactions are still largely unexplored because of limited access to human small intestinal tissues. Recently, we described an in vitro maturation technique for generating adult-like, mature human intestinal organoids (hIOs) from human pluripotent stem cells (hPSCs) that closely resemble the in vivo tissue structure and cellular diversity. Here, we established an in vitro human model to study the response to colonization by commensal bacteria using luminal microinjection into mature hIOs, allowing for the direct examination of epithelial-bacterial interactions.Lactobacillus reuteriandLactobacillus plantarumwere more likely to survive and colonize when microinjected into the lumen of mature hIOs than when injected into immature hIOs, as determined by scanning electron microscopy, colony formation assay, immunofluorescence, and real-time imaging withLplantarumexpressing red fluorescent protein. The improved mature hIO-based host epithelium system resulted from enhanced intestinal epithelial integrity via upregulation of mucus secretion and tight junction proteins. Our study indicates that mature hIOs are a physiologically relevant in vitro model system for studying commensal microorganisms. English WILEY ENTERICA SEROVAR TYPHIMURIUM BARRIER DISEASE HOST MICROBIOME MODEL BIFIDOBACTERIA DISRUPTION INTEGRITY HEALTH Maturation of human intestinal organoids in vitro facilitates colonization by commensal lactobacilli by reinforcing the mucus layer Article 10.1096/fj.202000063R 1 FASEB JOURNAL, v.34, no.8, pp.9899 - 9910 FASEB JOURNAL 34 8 9899 9910 scie scopus 000546040200001 2-s2.0-85087298556 Biochemistry & Molecular Biology Biology Cell Biology Biochemistry & Molecular Biology Life Sciences & Biomedicine - Other Topics Cell Biology Article ENTERICA SEROVAR TYPHIMURIUM BARRIER DISEASE HOST MICROBIOME MODEL BIFIDOBACTERIA DISRUPTION INTEGRITY HEALTH colonization host-microbe interaction human intestinal organoid lactobacilli microinjection mucus