Kim, Duck Gyun Yoo, Seok Woo Kim, Minsun Ko, Ja Kyong Um, Youngsoon Oh, Min-Kyu 2024-01-19T17:02:10Z 2024-01-19T17:02:10Z 2021-09-02 2020-08 0960-8524 https://pubs.kist.re.kr/handle/201004/118323 We previously engineered Enterobacter aerogenes for glucose and xylose co-utilization and 2,3-butanediol production. Here, strain EMY-22 was further engineered to improve the 2,3-butanediol titer, productivity, and yield by reducing the production of byproducts. To reduce succinate production, the budABC operon and galP gene were overexpressed, which increased 2,3-butanediol production. For further reduction of succinate and 2-ketogluconate production, maeA was selected and overexpressed in EMY-22. The optimally engineered strain produced 2,3-butanediol for a longer time and showed reduced byproduct formation from sugarcane bagasse hydrolysate under flask cultivation conditions. The engineered strain displayed 66.6, 13.4, and 16.8% improvements in titer, yield, productivity of 2,3-butanediol, respectively, compared to its parental strain under fed batch fermentation conditions. The data demonstrate that the metabolic engineering to reduce byproduct formation is a promising strategy to improve 2,3-butanediol production from lignocellulosic biomass. English Elsevier BV Improved 2,3-butanediol yield and productivity from lignocellulose biomass hydrolysate in metabolically engineered Enterobacter aerogenes Article 10.1016/j.biortech.2020.123386 1 Bioresource Technology, v.309 Bioresource Technology 309 N scie scopus 000569074800013 2-s2.0-85083460806 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article SUCCINIC ACID PRODUCTION CARBON CATABOLITE REPRESSION ESCHERICHIA-COLI ENHANCED PRODUCTION CITRATE SYNTHASE BINDING-SITE PRETREATMENT FERMENTATION GLUCOSE ENERGY Enterobacter aerogenes 2,3-butanediol Metabolic engineering Lignocellulosic biomass