Cho, Jinhong Park, Jinyoung Kim, Eunice EunKyeong Song, Eun Joo 2024-01-19T17:02:23Z 2024-01-19T17:02:23Z 2021-09-02 2020-08 1661-6596 https://pubs.kist.re.kr/handle/201004/118336 Deubiquitinating enzymes regulate various cellular processes, particularly protein degradation, localization, and protein-protein interactions. The dysregulation of deubiquitinating enzyme (DUB) activity has been linked to several diseases; however, the function of many DUBs has not been identified. Therefore, the development of methods to assess DUB activity is important to identify novel DUBs, characterize DUB selectivity, and profile dynamic DUB substrates. Here, we review various methods of evaluating DUB activity using cell lysates or purified DUBs, as well as the types of probes used in these methods. In addition, we introduce some techniques that can deliver DUB probes into the cells and cell-permeable activity-based probes to directly visualize and quantify DUB activity in live cells. This review could contribute to the development of DUB inhibitors by providing important information on the characteristics and applications of various probes used to evaluate and detect DUB activity in vitro and in vivo. English MDPI ACTIVITY-BASED PROBES FLUORESCENCE POLARIZATION DEUBIQUITYLATING ENZYMES ENZYMATIC-ACTIVITIES UBIQUITIN CANCER INHIBITOR IDENTIFICATION SPECIFICITY PROTEASES Assay Systems for Profiling Deubiquitinating Activity Article 10.3390/ijms21165638 1 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v.21, no.16 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21 16 scie scopus 000566404200001 2-s2.0-85089360226 Biochemistry & Molecular Biology Chemistry, Multidisciplinary Biochemistry & Molecular Biology Chemistry Review ACTIVITY-BASED PROBES FLUORESCENCE POLARIZATION DEUBIQUITYLATING ENZYMES ENZYMATIC-ACTIVITIES UBIQUITIN CANCER INHIBITOR IDENTIFICATION SPECIFICITY PROTEASES deubiquitination assay Ub-probes activity-based probes cell permeability