Jeon, Sangmin Park, Bum Chul Lim, Seungho Yoon, Hong Yeol Jeon, Yoo Sang Kim, Byung-Soo Kim, Young Keun Kim, Kwangmeyung 2024-01-19T17:02:41Z 2024-01-19T17:02:41Z 2021-09-04 2020-07-29 1944-8244 https://pubs.kist.re.kr/handle/201004/118353 The development of heat-generating magnetic nanostructures is critical for the effective management of tumors using magnetic hyperthermia. Herein, we demonstrate that polyethylene glycol (PEG)-coated iron oxide (magnetite, Fe3O4) multigranule nanoclusters (PEG-MGNCs) can enhance the efficiency of hyperthermia-based tumor suppression in vitro and in vivo. MGNCs consisting of granules (crystallites) measuring 22.9 nm in diameter were prepared via the hydrothermal polyol method, followed by the surface modification of MGNCs with PEG-dopamine. The freshly prepared PEG-MGNCs exhibit 145.9 +/- 10.2 nm diameter on average under aqueous conditions. The three-dimensional structures of PEG-MGNCs enhance the hyperthermic efficacy compared with PEGylated single iron-oxide nanoparticles (NPs), resulting in severe heat damage to tumor cells in vitro. In the SCC7 tumor-bearing mice, near-infrared fluorescence dye (Cy5.5)-labeled PEG-MGNCs are successfully accumulated in the tumor tissues because of NP-derived enhanced permeation and retention effect. Finally, the tumor growth is significantly suppressed in PEG-MGNC-treated mice after two-times heat generation by using a longitudinal solenoid, which can generate an alternating magnetic field under high-frequency (19.5 kA/m, 389 kHz) induction. This study shows for the first time that the PEG-MGNCs greatly enhance the hyperthermic efficacy of tumor treatment both in vitro and in vivo. English American Chemical Society MAGNETIC NANOPARTICLES SIZE DESIGN IMPACT MRI ACCUMULATION MECHANISMS PRINCIPLES EFFICIENCY NANOCUBES Heat-Generating Iron Oxide Multigranule Nanoclusters for Enhancing Hyperthermic Efficacy in Tumor Treatment Article 10.1021/acsami.0c07419 1 ACS Applied Materials & Interfaces, v.12, no.30, pp.33483 - 33491 ACS Applied Materials & Interfaces 12 30 33483 33491 scie scopus 000557854700004 2-s2.0-85089707778 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Science & Technology - Other Topics Materials Science Article MAGNETIC NANOPARTICLES SIZE DESIGN IMPACT MRI ACCUMULATION MECHANISMS PRINCIPLES EFFICIENCY NANOCUBES hyperthermia high frequency theranostic iron oxide nanoparticle multigranule nanocluster polyethylene glycol