Park, Hyo-Jung Bang, Eun-Kyoung Hong, Jung Joo Lee, Sang-Myeong Ko, Hae Li Kwak, Hye Won Park, Hyelim Kang, Kyung Won Kim, Rhoon-Ho Ryu, Seung Rok Kim, Green Oh, Hanseul Kim, Hye-Jung Lee, Kyuri Kim, Minjeong Kim, Soo Young Kim, Jae-Ouk El-Baz, Karim Lee, Hyukjin Song, Manki Jeong, Dae Gwin Keum, Gyochang Nam, Jae-Hwan 2024-01-19T17:03:29Z 2024-01-19T17:03:29Z 2021-09-04 2020-07 1433-7851 https://pubs.kist.re.kr/handle/201004/118399 As agonists of TLR7/8, single-stranded RNAs (ssRNAs) are safe and promising adjuvants that do not cause off-target effects or innate immune overactivation. However, low stability prevents them from mounting sufficient immune responses. This study evaluates the adjuvant effects of ssRNA derived from the cricket paralysis virus intergenic region internal ribosome entry site, formulated as nanoparticles with a coordinative amphiphile, containing a zinc/dipicolylamine complex moiety as a coordinative phosphate binder, as a stabilizer for RNA-based adjuvants. The nanoformulated ssRNA adjuvant was resistant to enzymatic degradation in vitro and in vivo, and that with a coordinative amphiphile bearing an oleyl group (CA-O) was approximately 100 nm, promoted effective recognition, and improved activation of antigen-presenting cells, leading to better induction of neutralizing antibodies following single immunization. Hence, CA-O may increase the efficacy of ssRNA-based adjuvants, proving useful to meet the urgent need for vaccines during pathogen outbreaks. English John Wiley & Sons Ltd. Nanoformulated Single-Stranded RNA-Based Adjuvant with a Coordinative Amphiphile as an Effective Stabilizer: Inducing Humoral Immune Response by Activation of Antigen-Presenting Cells Article 10.1002/anie.202002979 1 Angewandte Chemie International Edition, v.59, no.28, pp.11540 - 11549 Angewandte Chemie International Edition 59 28 11540 11549 N scie scopus 000530871600001 2-s2.0-85085142981 Chemistry, Multidisciplinary Chemistry Article NEUTRALIZATION TEST VACCINE ADJUVANTS SURFACE-CHARGE TLR TRANSLATION INTERFERON GENERATION AGONIST ELISA antigens nanoparticles RNA structures viruses amphiphiles