Choi, Ji-Woo Ju, Yeon Ha Choi, Yunsook Hyeon, Seung Jae Gadhe, Changdev G. Park, Jong-Hyun Kim, Mun Seok Baek, Seungyeop Kim, YoungSoo Park, Ki Duk Pae, Ae Nim Ryu, Hoon Lee, C. Justin Cho, Bong Rae 2024-01-19T17:30:40Z 2024-01-19T17:30:40Z 2021-09-04 2020-06-17 1948-7193 https://pubs.kist.re.kr/handle/201004/118516 Amyloid-beta (A beta) tracers have made a significant contribution to the treatment of Alzheimer's disease (AD) by allowing a definitive diagnosis in living patients. Unfortunately, they also detect tau and other protein aggregates that compromise test accuracy. In AD research, there has been a growing need for in vivo A beta imaging by two-photon microscopy, which enables deep-brain-fluorescence imaging. There is no suitable neuritic A beta probe for two-photon microscopy. Here we report PyrPeg, a novel two-photon fluorescent probe that can selectively target insoluble A beta rather than tau and alpha-synuclein aggregates in the AD model brain and postmortem brain. When injected intravenously, PyrPeg detects the neuritic plaques in the brain and olfactory bulb of the AD model. PyrPeg may serve as a useful blood-brain-barrier-penetrating diagnostic tool for optical and functional monitoring of insoluble forms of A beta aggregates in the living AD brain. English AMER CHEMICAL SOC AMYLOID-BETA PLAQUES FLUORESCENT-PROBE MULTIPHOTON MICROSCOPY DIAGNOSIS DYES PyrPeg, a Blood-Brain-Barrier-Penetrating Two-Photon Imaging Probe, Selectively Detects Neuritic Plaques, Not Tau Aggregates Article 10.1021/acschemneuro.0c00211 1 ACS CHEMICAL NEUROSCIENCE, v.11, no.12, pp.1801 - 1810 ACS CHEMICAL NEUROSCIENCE 11 12 1801 1810 scie scopus 000542930500014 2-s2.0-85086681763 Biochemistry & Molecular Biology Chemistry, Medicinal Neurosciences Biochemistry & Molecular Biology Pharmacology & Pharmacy Neurosciences & Neurology Article AMYLOID-BETA PLAQUES FLUORESCENT-PROBE MULTIPHOTON MICROSCOPY DIAGNOSIS DYES Two-photon imaging Alzheimer&apos s disease blood-brain barrier-penetrating neuritic plaques alpha-synuclein Tau protein