Kim, Han Young Kim, Tae Jung Kang, Lami Kim, Young-Ju Kang, Min Kyoung Kim, Jonghoon Ryu, Ju Hee Hyeon, Taeghwan Yoon, Byung-Woo Ko, Sang-Bae Kim, Byung-Soo 2024-01-19T17:32:08Z 2024-01-19T17:32:08Z 2021-09-04 2020-06 0142-9612 https://pubs.kist.re.kr/handle/201004/118597 Exosomes and extracellular nanovesicles (NV) derived from mesenchymal stem cells (MSC) may be used for the treatment of ischemic stroke owing to their multifaceted therapeutic benefits that include the induction of angiogenesis, anti-apoptosis, and anti-inflammation. However, the most serious drawback of using exosomes and NV for ischemic stroke is the poor targeting on the ischemic lesion of brain after systemic administration, thereby yielding a poor therapeutic outcome. In this study, we show that magnetic NV (MNV) derived from iron oxide nanoparticles (IONP)-harboring MSC can drastically improve the ischemic-lesion targeting and the therapeutic outcome. Because IONP stimulated expressions of therapeutic growth factors in the MSC, MNV contained greater amounts of those therapeutic molecules compared to NV derived from naive MSC. Following the systemic injection of MNV into transient middle-cerebral-artery-occlusion (MCAO)-induced rats, the magnetic navigation increased the MNV localization to the ischemic lesion by 5.1 times. The MNV injection and subsequent magnetic navigation promoted the anti-inflammatory response, angiogenesis, and anti-apoptosis in the ischemic brain lesion, thereby yielding a considerably decreased infarction volume and improved motor function. Overall, the proposed MNV approach may overcome the major drawback of the conventional MSC-exosome therapy or NV therapy for the treatment of ischemic stroke. English ELSEVIER SCI LTD PC12 CELLS ANGIOGENESIS EXOSOMES GROWTH DIFFERENTIATION BIODISTRIBUTION POLARIZATION MECHANISMS DELIVERY NANOPARTICLES Mesenchymal stem cell-derived magnetic extracellular nanovesicles for targeting and treatment of ischemic stroke Article 10.1016/j.biomaterials.2020.119942 1 BIOMATERIALS, v.243 BIOMATERIALS 243 scie scopus 000523565800011 2-s2.0-85081162683 Engineering, Biomedical Materials Science, Biomaterials Engineering Materials Science Article PC12 CELLS ANGIOGENESIS EXOSOMES GROWTH DIFFERENTIATION BIODISTRIBUTION POLARIZATION MECHANISMS DELIVERY NANOPARTICLES Extracellular nanovesicles Exosomes Iron oxide nanoparticles Mesenchymal stem cells Ischemic stroke