Yang, Yong Ryoul Kabir, Mohammad Humayun Park, Jin Hee Park, Jae-Il Kang, Jae Sook Ju, Shinyeong Shin, Yeo Jin Lee, Seung Min Lee, Jaemin Kim, Seokho Lee, Kwang-Pyo Lee, Soo Young Lee, Cheolju Kwon, Ki-Sun 2024-01-19T17:32:31Z 2024-01-19T17:32:31Z 2021-09-05 2020-05-15 1945-4589 https://pubs.kist.re.kr/handle/201004/118619 The blood exhibits a dynamic flux of proteins that are secreted by the tissues and cells of the body. To identify novel aging-related circulating proteins, we compared the plasma proteomic profiles of young and old mice using tandem mass spectrometry. The expression of 134 proteins differed between young and old mice. We selected seven proteins that were expressed at higher levels in young mice, and confirmed their plasma expression in immunoassays. The plasma levels of anthrax toxin receptor 2 (ANTXR2), cadherin-13 (CDH-13), scavenger receptor cysteine-rich type 1 protein M130 (CD163), cartilage oligomeric matrix protein (COMP), Dickkopf-related protein 3 (DKK3), periostin, and secretogranin-1 were all confirmed to decrease with age. We then investigated whether any of the secreted proteins influenced bone metabolism and found that CDH-13 inhibited osteoclast differentiation. CDH 13 treatment suppressed the receptor activator of NF-kappa B ligand (RANKL) signaling pathway in bone marrow-derived macrophages, and intraperitoneal administration of CDH-13 delayed age-related bone loss in the femurs of aged mice. These findings suggest that low plasma CDH-13 expression in aged mice promotes aging-associated osteopenia by facilitating excessive osteoclast formation. Thus, CDH-13 could have therapeutic potential as a protein drug for the prevention of osteopenia. English IMPACT JOURNALS LLC T-CADHERIN COGNITIVE FUNCTION ASSOCIATION GROWTH CDH13 DIFFERENTIATION PROGRESSION EXPRESSION CLEAVAGE CELLS Plasma proteomic profiling of young and old mice reveals cadherin-13 prevents age-related bone loss Article 10.18632/aging.103184 1 AGING-US, v.12, no.9, pp.8652 - 8668 AGING-US 12 9 8652 8668 scie scopus 000533150800064 2-s2.0-85085208059 Cell Biology Geriatrics & Gerontology Cell Biology Geriatrics & Gerontology Article T-CADHERIN COGNITIVE FUNCTION ASSOCIATION GROWTH CDH13 DIFFERENTIATION PROGRESSION EXPRESSION CLEAVAGE CELLS aging bone plasma proteins proteomics osteoclast differentiation