Kim, Gi-Cheon Lee, Choong-Gu Verma, Ravi Rudra, Dipayan Kim, Taemook Kang, Keunsoo Nam, Jong Hee Kim, Young Im, Sin-Hyeog Kwon, Ho-Keun 2024-01-19T17:32:32Z 2024-01-19T17:32:32Z 2021-09-05 2020-05-14 2234-943X https://pubs.kist.re.kr/handle/201004/118620 ETS1 has shown dichotomous roles as an oncogene and a tumor suppressor gene in diverse cancers, but its functionality in breast cancer tumorigenesis still remains unclear. We utilized the Cancer Genome Atlas (TCGA) database to analyze comprehensive functions of ETS1 in human breast cancer (BRCA) patients by investigating its expression patterns and methylation status in relation to clinical prognosis. ETS1 expression was significantly diminished by hyper-methylation of the ETS1 promoter region in specimens from BRCA patients compared to a healthy control group. Moreover, ETS1(high) BRCA patients showed better prognosis and longer survival compared to ETS1(low) BRCA patients. Consistent with clinical evidence, comparative transcriptome analysis combined with CRISPR/Cas9 or shRNA based perturbation of ETS1 expression revealed direct as well as indirect mechanisms of ETS1 that hinder tumorigenesis of BRCA cells. Taken together, our study enlightens a novel function of ETS1 as a tumor suppressor in breast cancer cells. English FRONTIERS MEDIA SA TRANSCRIPTION FACTOR PLASMINOGEN-ACTIVATOR CLINICAL-IMPLICATIONS DNA METHYLATION POOR-PROGNOSIS EXPRESSION SURVIVAL PROTOONCOGENE ANGIOGENESIS INVASION ETS1 Suppresses Tumorigenesis of Human Breast Cancer via Trans-Activation of Canonical Tumor Suppressor Genes Article 10.3389/fonc.2020.00642 1 FRONTIERS IN ONCOLOGY, v.10 FRONTIERS IN ONCOLOGY 10 scie scopus 000537840500001 2-s2.0-85085506410 Oncology Oncology Article TRANSCRIPTION FACTOR PLASMINOGEN-ACTIVATOR CLINICAL-IMPLICATIONS DNA METHYLATION POOR-PROGNOSIS EXPRESSION SURVIVAL PROTOONCOGENE ANGIOGENESIS INVASION ETS1 breast cancer tumor suppressor DNA methylation regulatory elements