Nam, G.-H. Pahk, K.J. Jeon, S. Park, H.-J. Kim, G.B. Oh, S.J. Kim, K. Kim, H. Yang, Y. 2024-01-19T17:32:59Z 2024-01-19T17:32:59Z 2022-01-28 2020-08 2366-3987 https://pubs.kist.re.kr/handle/201004/118644 Boiling histotripsy (BH) is a completely non-invasive ultrasonic technique that can be used to mechanically destroy tumor tissues. Studies have shown that BH has biological effects on immune responses, but the mechanisms involved in the induction and enhancement of systemic anti-tumor immune responses after BH treatment are poorly understood. The present study therefore investigates the anti-tumor immune responses triggered by BH exposure in vivo. In a syngeneic tumor model, BH treatment results in more dendritic cell maturation and increased intra-tumoral infiltration of activated CD8+ T？cells compared to those observed after thermal high-intensity focused ultrasound exposure. The results clearly show that tumor antigens and danger signals released after BH exposure can lead to a sufficient anti-tumor immune response with antigen-presenting cell activation. In the 4T1 triple-negative breast cancer model, BH-induced mechanical ablation further enhances the therapeutic effect of immune checkpoint blockade, indicating a synergistic anti-tumor immune effect. In conclusion, the results presented in this study suggest that BH is a promising option for boosting anti-tumor immunity and can be beneficially combined with cancer immunotherapy. ？ 2020 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim English Blackwell Publishing Ltd Investigation of the Potential Immunological Effects of Boiling Histotripsy for Cancer Treatment Article 10.1002/adtp.201900214 1 Advanced Therapeutics, v.3, no.8 Advanced Therapeutics 3 8 N scopus 000533522400001 2-s2.0-85103744447 Pharmacology & Pharmacy Pharmacology & Pharmacy Article calreticulin CD3 antigen chemokine receptor CXCR4 CXCL2 chemokine CXCL9 chemokine heat shock protein 70 high mobility group B1 protein immunological antineoplastic agent interleukin 16 interleukin 1alpha interleukin 1beta macrophage inflammatory protein 1alpha macrophage inflammatory protein 1beta tumor antigen tumor necrosis factor animal cell animal experiment animal model animal tissue antigen presenting cell antineoplastic activity Article boiling histotripsy bone marrow derived macrophage cancer growth cancer immunotherapy cancer infiltration cancer inhibition cancer model cancer recurrence CD8+ T lymphocyte cell maturation cell viability cellular immunity comparative study controlled study cytokine production dendritic cell enzyme linked immunosorbent assay flow cytometry high intensity focused ultrasound immune response in vivo study macrophage male mouse non invasive procedure nonhuman priority journal qualitative analysis T lymphocyte activation triple negative breast cancer tumor ablation tumor draining lymph node tumor immunity TUNEL assay ultrasound therapy boiling histotripsy cancer immunotherapy high-intensity focused ultrasound immune checkpoint blockade antigen-presenting cells