Kim, Jeonghyo Oh, Sangjin Shin, Yong Cheol Wang, Caifeng Kang, Moon Sung Lee, Jong Ho Yun, Woobin Cho, Jin Ah Hwang, Dae Youn Han, Dong-Wook Lee, Jaebeom 2024-01-19T17:33:08Z 2024-01-19T17:33:08Z 2022-01-25 2020-05 0927-7765 https://pubs.kist.re.kr/handle/201004/118652 From senescence and frailty that may result from various biological, mechanical, nutritional, and metabolic processes, the human body has its own antioxidant defense enzymes to remove by-products of oxygen metabolism, and if unregulated, can cause several types of cell damage. Herein, an antioxidant, artificial nanoscale enzyme, called nanozyme (NZs), is introduced that is composed of Au nanoparticles (NPs) synthesized with a mixture of two representative phytochemicals, namely, gallic acid (GA) and isoflavone (IF), referred to as GI-Au NZs. Their unique antioxidant and anti-aging effects are monitored using Cell Counting Kit-8 and senescence-associated beta-galactosidase assays on neonatal human dermal fibroblasts (nHDFs). Furthermore, alterations in epidermal thickness and SOD activity are measured under ultraviolet light to investigate the effects of the topical application of NZs on the histological structure and antioxidant activity in hairless mice skin. Then, hepatotoxicity and nephrotoxicity in the hairless mice are monitored. It is concluded that the NZs can effectively prevent serial passage-induced senescence in nHDFs, as well as oxidative stress in mice skin, suggesting a range of strategies to further develop novel therapeutics for acute frailty. English Elsevier BV Au nanozyme-driven antioxidation for preventing frailty Article 10.1016/j.colsurfb.2020.110839 1 Colloids and Surfaces B: Biointerfaces, v.189 Colloids and Surfaces B: Biointerfaces 189 N scie scopus 000527314700022 2-s2.0-85078908681 Biophysics Chemistry, Physical Materials Science, Biomaterials Biophysics Chemistry Materials Science Article GOLD NANOPARTICLES MECHANISMS CYTOTOXICITY GENERATION SKIN Phytochemical Au nanoparticle Anti-aging Frailty Senescence Nanozyme