Cha, Jin Wook Park, Sunghyouk 2024-01-19T17:33:59Z 2024-01-19T17:33:59Z 2021-08-31 2020-05 0003-2700 https://pubs.kist.re.kr/handle/201004/118702 In NMR analysis of complex organic molecules, low natural abundance of C-13 and the low resolution of two-dimensional (2D) experiments are significant difficulties. Also challenging is the analysis of a mixture spectrum without separation, which has been limited to simple molecules. Through nonuniform sampling using modified heteronuclear multiple bond correlation combined with indirect covariance, a high-resolution C-13-C-13 correlation spectrum was obtained with H-1 sensitivity. Built on the thus-obtained C-13-C-13 connectivities, deconvolution of the mixture spectra was achieved through a new signal-processing procedure, termed DECODE, tailored to the indirect covariance eigendecomposition. When applied to a complex natural product mixture of rotenone and brucine with many quaternary carbons, the method resolved very close carbon peaks and extracted clean individual spectra. Essentially providing molecule-wide C-13 connectivities for complex molecules from H-1-detected 2D spectra, our approach should prove useful in many areas of NMR analysis. English American Chemical Society Extraction of Individual Spectra from Mixture Data Based on High-Resolution C-13-C-13 NMR Correlation Spectrum and DECODE Procedure Article 10.1021/acs.analchem.0c00277 1 Analytical Chemistry, v.92, no.10, pp.7037 - 7044 Analytical Chemistry 92 10 7037 7044 N scie scopus 000537144800033 2-s2.0-85090298430 Chemistry, Analytical Chemistry Article CHEMICAL-MIXTURES COVARIANCE DECONVOLUTION ASSIGNMENT PROTEINS STRATEGY HMBC