Park, Ji Hye Son, Yang Ju Lee, Chang Ho Nho, Chu Won Yoo, Gyhye 2024-01-19T17:34:13Z 2024-01-19T17:34:13Z 2021-09-04 2020-04-22 2662-7671 https://pubs.kist.re.kr/handle/201004/118716 Background: Circaea mollis Sieb. & Zucc. has been used as a traditional herbal medicine in Hani Ethnopharmacy and possesses anti-arthritic activities. This study aimed to investigate the effect of Circaea mollis Siebold & Zucc on postmenopausal osteoporosis. Methods: For in vitro study, MCF7 breast cancer cells and MC3T3-E1 pre-osteoblast cells were utilized to estimate estrogenic and osteogenic activity. Osteoblastic markers were measured by western blot and real-time PCR. For in vivo study, female mature C57BL/6 mice were ovariectomized and oral administrated with 10 mg/kg and 40 mg/kg of EECM respectively. Results: EtOH extract of Circaea mollis Siebold & Zucc. (EECM) increased alkaline phosphatase activity and osteoblast marker levels at day 7 during differentiation of mouse preosteoblasts. EECM reduced osteoclast differentiation and bone resorption in an osteoblast-osteoclast primary co-culture system. In ovariectomized mice, EECM prevented the decrease in bone mineral density and recovered OSX and Runx2 via BMP2/4, Smad1/5/9 and p38. Conclusions: The results suggest that EECM may be effective in preventing bone loss, offering a promising alternative for the nutritional management of postmenopausal osteoporosis. English BMC ESTROGEN-RECEPTOR-ALPHA DIFFERENTIATION OSTEOBLAST EXPRESSION CELLS OSTEOCLASTS PREVENTION MECHANISMS INDUCTION Circaea mollis Siebold & Zucc. Alleviates postmenopausal osteoporosis in a mouse model via the BMP-2/4/Runx2 pathway Article 10.1186/s12906-020-02914-7 1 BMC COMPLEMENTARY MEDICINE AND THERAPIES, v.20, no.1 BMC COMPLEMENTARY MEDICINE AND THERAPIES 20 1 scie scopus 000550305300001 2-s2.0-85084268518 Integrative & Complementary Medicine Integrative & Complementary Medicine Article ESTROGEN-RECEPTOR-ALPHA DIFFERENTIATION OSTEOBLAST EXPRESSION CELLS OSTEOCLASTS PREVENTION MECHANISMS INDUCTION Circaea mollis Siebold & Zucc Postmenopausal osteoporosis Osteoblast differentiation Runt-related transcription factor 2 Bone morphogenetic protein 2/4