Selvaraj, Baskar Kim, Dae Won Huh, Gyuwon Lee, Heesu Kang, Kyungsu Lee, Jae Wook 2024-01-19T17:34:19Z 2024-01-19T17:34:19Z 2022-01-10 2020-04-15 0960-894X https://pubs.kist.re.kr/handle/201004/118721 Glutamate-induced neurotoxicity is characterized by cellular Ca2+ uptake, which is upstream of reactive oxygen species (ROS)-induced apoptosis signaling and MAPKs activation. In the present study, we synthesized isoliquiritigenin analogs with electron-donating and electron-withdrawing functional groups. These analogs were evaluated for neuroprotective effect against glutamate-induced neurotoxicity in HT22 cells. Among these analogs, compound BS11 was selected as a potent neuroprotective agent. Cellular Ca2+ concentration, ROS level, MAPKs activation and AIF translocation to the nucleus were increased upon treatment with 5 mM glutamate. In contrast, we identified that compound BS11 reduced the cellular Ca2+ concentration and ROS level upon glutamate exposure. Western blot analysis showed that MAPK activation was decreased by treatment with compound BS11. We further identified that cotreatment of compound BS11 and glutamate inhibited translocation of AIF to the nucleus. English Pergamon Press Ltd. OXIDATIVE STRESS NEURONAL CELLS APOPTOSIS PROTECTS INHIBITION TOXICITY NRF2 TRANSLOCATION ACTIVATION DEATH Synthesis and biological evaluation of isoliquiritigenin derivatives as a neuroprotective agent against glutamate mediated neurotoxicity in HT22 cells Article 10.1016/j.bmcl.2020.127058 1 Bioorganic & Medicinal Chemistry Letters, v.30, no.8 Bioorganic & Medicinal Chemistry Letters 30 8 scie scopus 000519194300022 2-s2.0-85080119608 Chemistry, Medicinal Chemistry, Organic Pharmacology & Pharmacy Chemistry Article OXIDATIVE STRESS NEURONAL CELLS APOPTOSIS PROTECTS INHIBITION TOXICITY NRF2 TRANSLOCATION ACTIVATION DEATH Isoliquirtigenin Glutamate induced neurotoxicity HT22 mouse hippocampal neuronal cells MAPKs AIF