Kim, Myungsuk Yoo, GyHye Randy, Ahmad Son, Yang-Ju Hong, Chi Rac Kim, Sang Min Nho, Chu Won 2024-01-19T18:00:28Z 2024-01-19T18:00:28Z 2021-09-05 2020-04 2072-6643 https://pubs.kist.re.kr/handle/201004/118786 Nonalcoholic fatty liver disease (NAFLD) ranges in severity from hepatic steatosis to cirrhosis. Lemon balm and its major constituent, rosmarinic acid (RA), effectively improve the liver injury and obesity; however, their therapeutic effects on nonalcoholic steatohepatitis (NASH) are unknown. In this study, we investigated the effects of RA and a lemon balm extract (LBE) on NAFLD and liver fibrosis and elucidated their mechanisms. Palmitic acid (PA)-exposed HepG2 cells and db/db mice fed a methionine- and choline-deficient (MCD) diet were utilized to exhibit symptoms of human NASH. LBE and RA treatments alleviated the oxidative stress by increasing antioxidant enzymes and modulated lipid metabolism-related gene expression by the activation of adenosine monophosphate-activated protein kinase (AMPK) in vitro and in vivo. LBE and RA treatments inhibited the expression of genes involved in hepatic fibrosis and inflammation in vitro and in vivo. Together, LBE and RA could improve liver damage by non-alcoholic lipid accumulation and may be promising medications to treat NASH. English MDPI IN-VITRO EPIGALLOCATECHIN GALLATE OXIDATIVE STRESS PPAR-ALPHA CELLS AMPK INFLAMMATION ACTIVATION STEATOSIS ACCUMULATION Lemon Balm and Its Constituent, Rosmarinic Acid, Alleviate Liver Damage in an Animal Model of Nonalcoholic Steatohepatitis Article 10.3390/nu12041166 1 NUTRIENTS, v.12, no.4 NUTRIENTS 12 4 scie scopus 000531831300286 2-s2.0-85083740783 Nutrition & Dietetics Nutrition & Dietetics Article IN-VITRO EPIGALLOCATECHIN GALLATE OXIDATIVE STRESS PPAR-ALPHA CELLS AMPK INFLAMMATION ACTIVATION STEATOSIS ACCUMULATION nonalcoholic steatohepatitis (NASH) lemon balm extract (LBE) rosmarinic acid (RA) AMP-activated protein kinase (AMPK) antioxidants anti-inflammation