Lee, Yu Ra Im, Eunju Kim, Haksoon Lew, Bark Lynn Sim, Woo-Young Lee, Jeongae Bin Oh, Han Paeng, Ki Jung Hong, Jongki Chung, Bong Chul 2024-01-19T18:00:56Z 2024-01-19T18:00:56Z 2021-09-05 2020-04 2218-1989 https://pubs.kist.re.kr/handle/201004/118812 Male pattern baldness (MPB) has been associated with dihydrotestosterone (DHT) expression. Finasteride treats MPB by inhibiting 5-alpha reductase and blocking DHT production. In this study, we aimed to identify metabolic differences in urinary metabolomics profiles between MPB patients after a one-year treatment with finasteride and healthy controls. Untargeted and targeted metabolomics profiling was performed using liquid chromatography-mass spectrometry (LC-MS). We hypothesized that there would be changes in overall metabolite concentrations, especially steroids, in the urine of hair loss patients treated with finasteride and normal subjects. Untargeted analysis indicated differences in steroid hormone biosynthesis. Therefore, we conducted targeted profiling for steroid hormone biosynthesis to identify potential biomarkers, especially androgens and estrogens. Our study confirmed the differences in the concentration of urinary androgens and estrogens between healthy controls and MPB patients. Moreover, the effect of finasteride was confirmed by the DHT/T ratio in urine samples of MPB patients. Our metabolomics approach provided insight into the physiological alterations in MPB patients who have been treated with finasteride for a year and provided evidence for the association of finasteride and estrogen levels. Through a targeted approach, our results suggest that urinary estrogens must be studied in relation to MPB and post-finasteride syndrome. English MDPI CEREBROSPINAL-FLUID HAIR MEN TESTOSTERONE PLASMA 5-ALPHA-REDUCTASE ANDROGENS Untargeted Metabolomics and Steroid Signatures in Urine of Male Pattern Baldness Patients after Finasteride Treatment for a Year Article 10.3390/metabo10040131 1 METABOLITES, v.10, no.4 METABOLITES 10 4 scie scopus 000533907300020 2-s2.0-85083240963 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article CEREBROSPINAL-FLUID HAIR MEN TESTOSTERONE PLASMA 5-ALPHA-REDUCTASE ANDROGENS male pattern baldness liquid chromatography-tandem mass spectrometry androgens estrogen finasteride