Kang, Li-Jung Oh, Eunjeong Cho, Chanmi Kwon, HoKeun Lee, Choong-Gu Jeon, Jimin Lee, Hyemi Choi, Sangil Han, Seong Jae Nam, Jiho Song, Chi-une Jung, Hyunho Kim, Hye Young Park, Eun-Jung Choi, Eun-Ju Kim, Jooyoung Eyun, Seong-il Yang, Siyoung 2024-01-19T18:01:19Z 2024-01-19T18:01:19Z 2021-09-05 2020-03-27 2045-2322 https://pubs.kist.re.kr/handle/201004/118834 3 ' -Sialyllactose (3 ' -SL), a natural prebiotic, maintains immune homeostasis and exerts anti-inflammatory and anti-arthritic effects. Although regulatory T cells (Tregs) prevent excessive inflammation and maintain immune tolerance, the effect of 3 ' -SL on Treg regulation is unclear. This study aimed to investigate the effect of 3 ' -SL on Treg responses in atopic dermatitis (AD) pathogenesis. Oral administration of 3 ' -SL reduced AD-like symptoms such as ear, epidermal, and dermal thickness in repeated topical application of house dust mites (HDM) and 2,4-dinitrochlorobenzene (DNCB). 3 ' -SL inhibited IgE, IL-1 beta, IL-6, and TNF-alpha secretion and markedly downregulated AD-related cytokines including IL-4, IL-5, IL-6, IL-13, IL-17, IFN-gamma, TNF-alpha, and Tslp through regulation of NF-kappa B in ear tissue. Additionally, in vitro assessment of Treg differentiation revealed that 3 ' -SL directly induced TGF-beta -mediated Treg differentiation. Furthermore, 3 ' -SL administration also ameliorated sensitization and elicitation of AD pathogenesis by suppressing mast cell infiltration and production of IgE and pro-inflammatory cytokines in mouse serum by mediating the Treg response. Furthermore, Bifidobacterium population was also increased by 3 ' -SL administration as prebiotics. Our data collectively show that 3 ' -SL has therapeutic effects against AD progression by inducing Treg differentiation, downregulating AD-related cytokines, and increasing the Bifidobacterium population. English NATURE PUBLISHING GROUP THYMIC STROMAL LYMPHOPOIETIN NF-KAPPA-B MAST-CELLS MECHANISMS RESPONSES IGE 3 '-Sialyllactose prebiotics prevents skin inflammation via regulatory T cell differentiation in atopic dermatitis mouse models Article 10.1038/s41598-020-62527-5 1 SCIENTIFIC REPORTS, v.10, no.1 SCIENTIFIC REPORTS 10 1 scie scopus 000560407600008 2-s2.0-85082472958 Multidisciplinary Sciences Science & Technology - Other Topics Article THYMIC STROMAL LYMPHOPOIETIN NF-KAPPA-B MAST-CELLS MECHANISMS RESPONSES IGE Prebiotics Skin inflammation Regulatory T cells Atopic dermatitis Microbiome Gut