Lee, Inae Kim, Seong-Eun Lee, Jiho Woo, Deok Ha Lee, Seok Pyo, Heesoo Song, Chang-Seon Lee, Joonseok 2024-01-19T18:01:38Z 2024-01-19T18:01:38Z 2021-09-05 2020-03-15 0956-5663 https://pubs.kist.re.kr/handle/201004/118851 Conventional electrochemical biosensing systems rely on a single output signal, which limits their certain practical application, specifically from the viewpoint of external interference factors causing electrochemical signal errors. This study reports a self-calibrating dual-electrode based electrochemical aptasensor for the reliable and independent detection of avian influenza viruses (ATVs), which are the primary cause of highly contagious respiratory diseases, under external interference factors. Both electrodes were fabricated using tungsten rods surface-modified with a 3D nanostructured porous silica film (3DNRE). Subsequently, methylene blue (MB) was loaded as a redox-active material into the pores and capped with corresponding aptamer. One electrode was capped with an anti-AIV nucleoprotein (NP) aptamer (Apt(AIV)-MB@3DNRE) allowing target-specific binding, resulting in changes in electrochemical signal upon diffusional release of the loaded redox molecules. The other electrode was capped with a control aptamer (Apt(con)-MB@3DNFRE), serving as a reference to correct false responses generated by nonspecific aptamer detachment and MB release under environmental changes in pH and ion strength and presence of nontarget molecules from cell lysis debris. In the dual-electrode platform, Apt(con)-MB@3DNFRE provides a corrected baseline for the fluctuating original output signals from Apt Apt(AIV)-MB@3DNRE. Consequently, this dual-electrode platform exhibits excellent output-signal stability (relative standard deviation, RSD: 5.86%) compared to a conventional single-electrode platform (RSD: 30.13%) at equivalent concentrations of AIV NP samples under different reaction buffer conditions. Moreover, no further purification and washing steps were required, indicating that the strategy may represent a universal and reliable platform for the electrochemical aptamer-based detection of various biomolecules. English ELSEVIER ADVANCED TECHNOLOGY LABEL-FREE PROTEIN DNA IMMUNOASSAY BIOSENSORS APTAMERS BINDING COPPER A self-calibrating electrochemical aptasensing platform: Correcting external interference errors for the reliable and stable detection of avian influenza viruses Article 10.1016/j.bios.2020.112010 1 BIOSENSORS & BIOELECTRONICS, v.152 BIOSENSORS & BIOELECTRONICS 152 scie scopus 000515210600006 2-s2.0-85077514028 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article LABEL-FREE PROTEIN DNA IMMUNOASSAY BIOSENSORS APTAMERS BINDING COPPER Self-calibrating Dual electrode Aptasensor Avian influenza viruses 3D nanostructures